Imaging the Gel
Detection of bound probe
After electrophoresis of the protein:DNA complexes, detection is performed.
- If an IRDye® fluorescent probe is used, binding results are imaged immediately by scanning the wet gel.
- Acrylamide gels can be scanned with the glass plates still in place.
- Gels can be returned to the electrophoresis unit and run longer, if desired.
- If a radioactive probe is used, the gel is dried and exposed to X-ray film to document the binding results.