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Imaging the Gel

Detection of bound probe

After electrophoresis of the protein:DNA complexes, detection is performed.

  • If an IRDye® fluorescent probe is used, binding results are imaged immediately by scanning the wet gel.
    • Acrylamide gels can be scanned with the glass plates still in place.
    • Gels can be returned to the electrophoresis unit and run longer, if desired.
  • If a radioactive probe is used, the gel is dried and exposed to X-ray film to document the binding results.

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