EMSA/gel shift assay. When a large molar excess of unlabeled competitor DNA is added, the mobility shift is greatly reduced. Fluorescently labeled IRDye 700 oligonucleotide probes are used for detection. IRDye 700 oligos are end-labeled on both strands.
The EMSA (electrophoretic mobility shift assay) is used to study protein:DNA complexes and interactions. Protein:DNA complexes migrate more slowly than unbound linear DNA on a non-denaturing gel, causing a "shift."
Also called "gel shift" or "gel retardation" assays, EMSA can be used to analyze sequence-specific recognition of nucleic acids by proteins.
Traditional, radioactive EMSA protocols can be easily adapted to near-infrared fluorescence EMSA detection by using IRDye® end-labeled oligonucleotides and imaging with the Odyssey® CLx or Odyssey Classic Infrared Imaging System, providing a safe and sensitive alternative.
EMSAs using near-infrared fluorescence technology are used to study:
Compare EMSA detection methods and find out how you can increase safety and save time using infrared detection.