|Auto Sensitivity selected in Odyssey Software
||Change sensitivity setting to manual and adjust Sensitivity manually. Use Image Studio software and the Auto Scan mode.
|Scanned gel with intensity too low
||Increase intensity parameter in Odyssey software to 8 and scan again. Use Image Studio software and the Auto Scan mode.
|Incorrect focus offset
||Adjust the Focus Offset in the Odyssey software or Image Studio software to equal the thickness of the glass plate plus half the thickness of the gel, and scan again.
|DNA:protein complex disrupted due to heat or vortexing
||Run gel with cooled buffer. Do not vortex binding reaction.
|Not enough extract
||Add more extract to reaction.
||Minimize freeze/thaw cycles. Use protease inhibitors.
|System not fully optimized
||Use additives in the kit (Odyssey EMSA Buffer Kit, P/N 829-07910) to determine their effects on binding efficiency.