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Glycoprotein Antibody Detection

Description:

O-GlcNAc (O-linked N-acetylglucosamine) modifications can be detected using a specific antibody and IRDye® secondary antibodies.

Detection Cartoon:

Slide 18

Data:

Slide 19

α-crystallin was detected using O-GlycNAc mouse monoclonal antibody and a IRDye 800CW Goat anti-mouse secondary. The blot was imaged on the Odyssey.



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