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Enzymatic Glycoprotein Detection

Description:

O-linked glycosylated proteins can be modified with the Click-iT™ O-GlcNAc enzymatic labeling kit and detected using the Click-IT™ Biotin Glycoprotein Detection Kit in combination with IRDye® Streptavidin.

Detection Cartoon:

Enzymatic Glyco Detection

Data:

Slide 21

α-Crystallin was labeled via Click-iT™ chemistry using Molecular Probes enzymatic O-GlcNAc labeling kit. The modified and unmodified products were resolved on a gel and transferred to a nitrocellulose membrane.  The IRDye 800CW Streptavidin (P/N 926-32230) was used to detect the modified α-crystallin product by Western blot.



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