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Normalization to Cell Number:
CellTag™ 700 Staining

fig1DNAstaining

Figure 1. Linear Relationship between Fluorescence and Cell Number. Two-fold serial dilutions of A431 or NIH/3T3 cells were plated in 96-well plates. Cells were fixed, permeabilized, stained with CellTag 700 Stain (0.2 µM), and detected with Odyssey® Classic Infrared Imaging System. The Trim Signals were used to generate the graphs.

Cell number normalization is a fast and inexpensive approach, because no additional antibodies are required. Options include CellTag 700 staining and cell labeling with reactive dye.

CellTag 700 staining

  • CellTag 700 Stain is a near-infrared fluorescent cell stain that provides accurate information to cell number for In-Cell Western™ applications
  • The stain accumulates in both the nucleus and the cytoplasm of permeabilized cells and provides linear fluorescent signal across a wide range of cell types and cell membranes (Fig. 1)
  • Fast and easy - staining is combined with secondary antibody incubation

1. Hoffman, GR et al. Assay Drug Dev Tech 8(2): 186-99 (2010)

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