Figure 1. Linear Relationship between Fluorescence and Cell Number. Two-fold serial dilutions of A431 or NIH/3T3 cells were plated in 96-well plates. Cells were fixed, permeabilized, stained with CellTag 700 Stain (0.2 µM), and detected with Odyssey® Classic Infrared Imaging System. The Trim Signals were used to generate the graphs.
Cell number normalization is a fast and inexpensive approach, because no additional antibodies are required. Options include CellTag 700 staining and cell labeling with reactive dye.
1. Hoffman, GR et al. Assay Drug Dev Tech 8(2): 186-99 (2010)Back to Main Page