Half-maximal activation (EC50) of cellular β-catenin levels occurs at 33 ng/ml ligand.
Hannoush, RN. PLoS One 3(10):e3498 (2008). Creative Commons license 2.5.
A) HeLa cells were treated with increasing amounts of rapamycin in a 384- well format. Fixed cells were stained with phospho-rpS6 antibody and NHS-ester reactive dye (for cell number). Dose dependent inhibition of phospho-rpS6-staining yielded an IC50 of 224 pM (n=4). B) Raw microplate image.
Hoffman, GR et al. Assay Drug Dev Tech 8(2):186-99 (2010)