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Blocking Buffers

No single blocking reagent will be optimal for every antigen-antibody pair. Some primary antibodies may exhibit greatly reduced signal or different non-specific binding in different blocking solutions.

  • If you have difficulty detecting your target protein, changing the blocking buffer may improve performance.
  • If the primary antibody has worked well for immunofluorescent staining, try the same blocking buffer for ICW detection.
  • Odyssey® Blocking Buffer works well for many target proteins in ICW assays.


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