No single blocking reagent will be optimal for every antigen-antibody pair. Some primary antibodies may exhibit greatly reduced signal or different non-specific binding in different blocking solutions.
- If you have difficulty detecting your target protein, changing the blocking buffer may improve performance.
- If the primary antibody has worked well for immunofluorescent staining, try the same blocking buffer for ICW detection.
- Odyssey® Blocking Buffer works well for many target proteins in ICW assays.