Chat With Us

Fixation Conditions

fig1Fixation

No single fixative is appropriate for every cell line or target protein, so optimal conditions should be determined empirically.

Two general classes of fixative can be used:

  • Cross-linking reagents
    • Cross-linkers (usually aldehydes, such as formaldehyde) form a network of intermolecular bridges by linking antigens through free reactive groups, primarily amino groups.
    • Cross-linking helps to preserve cell structure.
    • A separate step is usually required to permeabilize the cell membrane.
    • Cross-linking reagents may denature some proteins and reduce their antigenicity.
  • Organic solvents
    • Organic solvents dehydrate the cell. This precipitates cellular proteins and causes cells to adhere to the plastic surface of the microplate.
    • A separate permeabilization step may not be needed.
    • Organics may denature or damage some proteins, and severely reduce their antigenicity.


Scroll to Top