A) Cultured cells (HeLa, HepG2, H196, HL-60, or Jurkat) were fixed and parallel samples were analyzed by ICW. Bak, Bax, Bcl-XL, and Mcl-1 antibodies were used. B) Mean and standard error of the mean (n=3) of the antibody-specific signal (normalized to cell number) is shown. Source: Mitosciences (In-Cell ELISA validated antibodies for apoptosis)
Mouse L-cells were treated with Wnt3a (50 ng/ml) in the absence or presence of either cycloheximide (CHX, 100 μg/ml) or actinomycin D (ActD, 1 μM) and incubated for the indicated times (n = 4).
Hannoush, RN. PLoS One 3(10):e3498 (2008). doi:10.1371/journal.pone.0003498.g003. Creative Commons license 2.5.