IMPORTANT: Use the same fixation and staining conditions that will be used for ICW Assays!
Figure 1. PARP-1 (cleaved) antibody specificity in apoptotic cells. Immunocytochemical analysis of HeLa cells, either untreated or treated with 1 μM Staurosporine for 4 h to induce apoptosis. Mitosciences PARP-1 (cleaved) In-Cell ELISA Kit (IR) (ab110215) was used. The PARP-1 (cleaved) antibody stains cell nuclei, but only after STS treatment.
Fluorescent signals can be assessed by near-infrared fluorescence microscopy, or microplate assays imaged with the Odyssey® Classic, CLx, or Sa Imagers.
1. Aguilar HN et al. PLoS ONE 5(4): e9965 (2010)