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Optimization of Fixation and Permeabilization Conditions

For In-Cell Western assays, cells must be immobilized onto the microplate surface and antigens must be made available to their antibodies. For optimal performance:

  • Identify the most appropriate fixative for your protein target
  • Start with conditions that work for immunofluorescent (IF) staining of that target
  • Follow IF recommendations from the primary antibody vendor
  • Read more about permeabilization


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