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Correlation of In-Cell Western Assays with Other Assays

In-Cell Western assay results correlate well with immunoblot results and other assays.

Compared to Western blotting, ICW assays display:

  • Very similar profiles of signal increase and decrease, and similar IC50 values
  • Biologically and physiologically validated data output1
  • Improved reproducibility and precision1
    • Significantly smaller standard deviations
    • Replicate measurements with very low CVs
  • Higher throughput, to easily process many samples or replicates in parallel

1. Aguilar HN et al. PLoS ONE 5(4): e9965 (2010)



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