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Protein Phosphorylation: Effects of Drug Compounds on Signaling Pathways


In-Cell Western™ assay was used to measure effects of the MEK inhibitor U0126 on EGFR-induced signaling.

A431 cells were treated with U0126 and stimulated with EGF. Phosphorylation of EGFR, ERK, and Stat3 was then measured. As expected, only ERK phosphorylation was inhibited. The IC50 was consistent with published reports.

Chen H et al. Anal Biochem 338: 136-142 (2005)

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