Proper selection of microplates can significantly affect results, as each plate has its own characteristics, including well depth, plate autofluorescence, and well-to-well signal crossover. We provide some general considerations for microplate selection and focus offset optimization to ensure the best imaging and results.
Selection of the right blocking buffer for your experiment is critical for optimal results. We provide a few suggestions and guidelines on blocking buffer selection.
You can use cells in suspension for In-Cell Western assays. Suggested protocols are provided for using suspension cells in In-Cell Western assays.