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FAQs and Tech Tips

  • Plate Selection & Instrument Settings

    Proper selection of microplates can significantly affect results, as each plate has its own characteristics, including well depth, plate autofluorescence, and well-to-well signal crossover. We provide some general considerations for microplate selection and focus offset optimization to ensure the best imaging and results.

  • Positioning Microplates
  • Blocking Buffers

    Selection of the right blocking buffer for your experiment is critical for optimal results. We provide a few suggestions and guidelines on blocking buffer selection.

  • Using Cells in Suspension

    You can use cells in suspension for In-Cell Western assays. Suggested protocols are provided for using suspension cells in In-Cell Western assays.

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