Microwestern arrays are a systems biology approach for quantitative multiplex analysis of cell signaling pathways and can be performed on the Odyssey® CLx or Odyssey Classic Imaging System.
Mark F Ciaccio, Joel P Wagner, Chih-Pin Chuu, Douglas A Lauffenburger & Richard B Jones
The Ben May Department for Cancer Research and the Institute for Genomics and Systems Biology, The University of Chicago, Chicago, Illinois. Center for Cell Decision Processes and Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, Present address: Institute of Cellular and Systems Medicine, National Health Research Institutes, Miaoli County, Taiwan.
Ciaccio et al. developed a method for doing higher throughput Western blotting using 200-fold less protein sample and antibody called MicroWestern Arrays (MWAs). MWAs have the scalability of reverse phase arrays yet they retain the vital attributes of western blots where signals that can be related to protein size. The method should be useful for analysis of proteins from cell lines and tissues in an analogous manner to spotted DNA microarrays for interrogation but with the user's choice of antibodies. Protein samples are spotted on acrylamide slabs in a 96 well array, electophoresed, transferred to a membrane, placed in 96-well gasket device and imaged on the Odyssey® Classic Infrared Imaging System. The ability to obtain information regarding hundreds of proteins with the MWAs should allow advances in our understanding of cell context-specific networks underlying human disease when combined with approppriate computational modeling methods.Learn more at Nature.com
Search our database for relevant peer-reviewed journal citations.Search Publications
Huang, J-H., et al.
CR3 and Dectin-1 Collaborate in Macrophage Cytokine Response through Association on Lipid Rafts and Activation of Syk-JNK-AP-1 Pathway
PLoS Pathog., Jul 2015; 11: e1004985.
Ciaccio, M.F., et al.
The DIONESUS algorithm provides scalable and accurate reconstruction of dynamic phosphoproteomic networks to reveal new drug targets.
Integr. Biol. (Camb.), Jul 2015; 7: 776–791.
Lin, H-P., et al.
Caffeic acid phenethyl ester induced cell cycle arrest and growth inhibition in androgen-independent prostate cancer cells via regulation of Skp2, p53, p21Cip1 and p27Kip1
Oncotarget, Mar 2015; 6: 6684–6707.
Hause, R.J., et al.
Identification and Validation of Genetic Variants that Influence Transcription Factor and Cell Signaling Protein Levels
Am. J. Hum. Genet., Aug 2014; 95: 194–208.
Lin, Y-C., et al.
Bovine Induced Pluripotent Stem Cells Are More Resistant to Apoptosis than Testicular Cells in Response to Mono-(2-ethylhexyl) Phthalate
Int. J. Mol. Sci., Mar 2014; 15: 5011–5031.
Wang, S-W., et al.
Androgen receptor-mediated apoptosis in bovine testicular induced pluripotent stem cells in response to phthalate esters
Cell Death Dis., Nov 2013; 4: e907.
Chuu, C-P., et al.
Caffeic acid phenethyl ester suppresses the proliferation of human prostate cancer cells through inhibition of p70S6K and Akt signaling networks.
Cancer Prev Res (Phila)., May 2012; 5: 788–797.
Liu, J., et al.
Effect of complementary pathway blockade on efficacy of combination enzastaurin and rapamycin.
Head Neck, Dec 2011; 33: 1774–1782.
Chevrier, N., et al.
Systematic Discovery of TLR Signaling Components Delineates Viral-Sensing Circuits
Cell, Nov 2011; 147: 853–867.
Ciaccio, M.F., et al.
Systems Analysis of EGF Receptor Signaling Dynamics with Micro-Western Arrays
Nat. Methods, Feb 2010; 7: 148–155.