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EtBr Imaging

fig1-etbr_imaging

Figure 1.

Sensitivity is high and background fluorescence is low when using SYTO® 60 red fluorescent nucleic acid stain to visualize DNA gels on the LI‑COR Odyssey® Infrared Imagers. The detection sensitivity and lower limit of detection for SYTO 60 with any of the Odyssey Imagers are better than those of ethidium bromide detected with either a Polaroid camera or a CCD imaging system1.

If you need to image ethidium bromide, SYBR® Safe DNA stains, or other DNA stains in your nucleic acid gels in the 600 nm region, use the Odyssey® Fc Imaging System.

Comparison of detection limits between ethidium bromide (Polaroid of CCD camera) and SYTO 60 (Odyssey Infrared Imager).

Table 1. SYTO 60: Limit of Detection (LOD) with Odyssey Imaging

DNA Fragment (bp) LOD (pg) LOD (fmol)
100 375 5.7
500 95 0.29
1500 44 0.044

Table 2. Ethidium Bromide: Limit of Detection (LOD) with Polaroid Imaging

DNA Fragment (bp) LOD (pg) LOD (fmol)
100 3000 45
500 1500 4.5
1500 700 0.7

Table 3. Ethidium Bromide: Limit of Detection (LOD) with CCD Imaging

DNA Fragment (bp) LOD (pg) LOD (fmol)
100 750 11.4
500 375 1.14
1500 175 0.18

1. Syto 60 Staining of Nucleic Acids on Gels LI-COR Biosciences (2010)



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