Figure 1. Time course of JNK phosphorylation, in response to LPS treatment. Phospho-JNK is shown in green, total JNK in red. Yellow indicates overlapping signals. Reprinted with permission from Bond, D. et al. Biol Proced Online 10(1): 20-28 (2008).
Use infrared fluorescence, IRDye® secondary antibodies, and Odyssey® imagers to detect Western blots without film, a darkroom, or any chemiluminescent substrates.