Accurate Western Blot Quantification
Figure 1. β-catenin, c-Myc, and CtBP1 are upregulated in colon cancer cell lines of increasing tumorigenicity. Gujral et al (2010). PLoS ONE 5(4): e10024. doi:10.1371/journal.pone.0010024.g001.
Infrared fluorescent signals are directly proportional to the amount of antigen on Odyssey® Western blots.
- Accurately and reproducibly measure changes in protein levels, with ratiometric detection (Fig. 1)
- Use IRDye® secondary antibodies and the Odyssey Imager for direct, non-enzymatic detection
- Detect both faint and strong bands accurately, in a single image, with excellent sensitivity
- Multiplex to detect two targets simultaneously
- Stable fluorescent signals allow you to store blots and re-image later
With Odyssey Westerns, signals do not saturate easily and the quantitative range is extremely wide.
- Film and chemiluminescence saturate quickly and have a limited quantitative range
- Direct comparison demonstrates that Odyssey detection provides a wider dynamic range than ECL and film1
- Western blot detection of Hsp70 is reported to be quantitative across 4.3 orders of magnitude, from 5 pg to 100 ng2
1. Wang et al. PNAS 104(30): 12365-70 (2007)
2. Bromage, E et. al. Marine Ecol Prog Ser 376: 123-132 (2009)