Accurately Quantitate Western Blots with Infrared Fluorescence

Accurate Western Blot Quantification

b-catenin, c-Myc, and CtBP1 are upregulated in colon cancer cell lines of increasing tumorigenicity.

Figure 1. β-catenin, c-Myc, and CtBP1 are upregulated in colon cancer cell lines of increasing tumorigenicity. Gujral et al (2010). PLoS ONE 5(4): e10024. doi:10.1371/journal.pone.0010024.g001.

Infrared fluorescent signals are directly proportional to the amount of antigen on Odyssey® Western blots.

  • Accurately and reproducibly measure changes in protein levels, with ratiometric detection (Fig. 1)
  • Use IRDye® secondary antibodies and the Odyssey Imager for direct, non-enzymatic detection
  • Detect both faint and strong bands accurately, in a single image, with excellent sensitivity
  • Multiplex to detect two targets simultaneously
  • Stable fluorescent signals allow you to store blots and re-image later

With Odyssey Westerns, signals do not saturate easily and the quantitative range is extremely wide.

  • Film and chemiluminescence saturate quickly and have a limited quantitative range
  • Direct comparison demonstrates that Odyssey detection provides a wider dynamic range than ECL and film1
  • Western blot detection of Hsp70 is reported to be quantitative across 4.3 orders of magnitude, from 5 pg to 100 ng2

Image Studio Features and Benefits Video

  • 1. Wang et al. PNAS 104(30): 12365-70 (2007)

  • 2. Bromage, E et. al. Marine Ecol Prog Ser 376: 123-132 (2009)



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