Figure 1. β-gal activity assay using DDAOG as substrate. HEK293 cells were transiently transfected with either a β-gal expressing vector (CMV-gal) or control vector. The signal intensity produced by DDAOG cleavage is linearly related to the substrate concentration. Signal intensities obtained using different concentrations of DDAOG (μM) were determined by imaging in the Odyssey 700 nm channel. The original image (A) and quantified data (B) are shown.
The β-galactosidase (β-gal) reporter gene is widely used in genetics and molecular biology. Uses include:
β-gal assays can be performed directly with the Odyssey® CLx, Odyssey Classic, or Odyssey Sa Infrared Imager in microplate format, using the near-infrared fluorescent substrate DDAOG (9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) β-D-galactopyranoside). The cleaved substrate fluoresces strongly in the 700 nm channel. This assay was published in Analytical Biochemistry in 2009.3