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Technical Tips

Odyssey CLx
  • Different lots or shipments of microplates may vary in the background fluorescence of the plastic. It is wise to check the background fluorescence levels of a new batch of plates by imaging an empty plate before running the assays.
  • If the β-gal expression is low, use a higher DDAOG concentration (e.g., 50 μM, or a 1:200 dilution of the DDAOG stock solution in Lysis and Reaction Buffer).
  • When the optimum incubation time has been determined, the enzymatic reaction can be stopped with stop buffer if desired (add NaCO3 to a final concentration of 0.33 M).
  • When pipetting, be careful not to introduce bubbles. Bubbles in the wells may refract light during imaging.

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