Search our database for relevant peer-reviewed journal citations.
Listed below are recent publications in which authors have used reverse phase lysate arrays imaged on the Odyssey® Imaging Systems for a wide range of research areas.
- Negm , O.H., et al.
Clinical utility of reverse phase protein array for molecular classification of breast cancer
Breast Cancer Res. Treat., Jan 2016; 155: 25–35.
Application: Quantitative analysis of samples extracted from formalin-fixed breast cancer tissues using IRDye® Secondary Antibodies. Comparisons made with standard immunohistochemistry.
- Greco, E., corresponding author Aita, A., et al.
The novel S59P mutation in the TNFRSF1A gene identified in an adult onset TNF receptor associated periodic syndrome (TRAPS) constitutively activates NF-κB pathway
Arthritis Res. Ther., Apr 2015; 17: 93.
Application: Quantitative analysis of the NF-B signaling pathway in HEK-293 cells transfected with wild-type and mutant TNFRSF1A cDNA vectors. Signals detected with IRDye® Secondary Antibodies.
- Sonntag, J., et al.
Reverse phase protein array based tumor profiling identifies a biomarker signature for risk classification of hormone receptor-positive breast cancer
Transl. Proteom., March 2014; 2: 52–59.
Application: Quantitative analysis of tumor lysates from human patients diagnosed with invasive breast carcinoma. Fast Green FCF staining and imaging used for normalization of array signals to total protein.
- O'Mahony, F.C., et al.
The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers
J. Vis. Exp., Jan 2013; (71): 50221.
Application: Quantitative analysis of lysates from tumors using Odyssey® Blocking Buffer (PBS) and IRDye®Secondary Antibodies, with a video demonstration.
- Luckert, K., et al.
A Dual Array-Based Approach to Assess the Abundance and Posttranslational Modification State of Signaling Proteins
Sci. Signal., Jan 2012; 5(206): pl1.
Application: Quantitative analysis of the Wnt signaling pathway in HepG2 cells using Odyssey® Blocking Buffer (PBS) and IRDye® Secondary Antibodies.
- Aleskandarany, M.A., et al.
The prognostic significance of STAT3 in invasive breast cancer: analysis of protein and mRNA expressions in large cohorts
Breast Cancer Res. Treat., Feb 2016; 156: 9–20.
- Fallahi-Sichani, M., et al.
Systematic analysis of BRAFV600E melanomas reveals a role for JNK/c-Jun pathway in adaptive resistance to drug-induced apoptosis
Mol. Syst. Biol., Mar 2015; 11: 0797.
- Jerjees, D.A., et al.
The mammalian target of rapamycin complex 1 (mTORC1) in breast cancer: the impact of oestrogen receptor and HER2 pathways.
Breast Cancer Res. Treat., Feb 2015; 150: 91–103.
- Pandey, V., et al.
Comparative Analysis and Modeling of the Severity of Steatohepatitis in DDC-Treated Mouse Strains
PLoS One, Oct 2014; 9: e111006.
- Negm, O.H., et al.
A pro-inflammatory signalome is constitutively activated by C33Y mutant TNF receptor 1 in TNF receptor-associated periodic syndrome (TRAPS)
Eur. J. Immunol., Jul 2014; 44: 2096–2110.
- Aleskandarany , M.A., et al.
Epithelial mesenchymal transition in early invasive breast cancer: an immunohistochemical and reverse phase protein array study.
Breast Cancer Res. Treat., Jun 2014; 145: 339–348.
- Goltsov, A., et al.
Systems Analysis of Drug-Induced Receptor Tyrosine Kinase Reprogramming Following Targeted Mono- and Combination Anti-Cancer Therapy
Cells, Jun 2014; 3: 563–591.
- Ummanni, R., et al.
Evaluation of reverse phase protein array (RPPA)-based pathway-activation profiling in 84 non-small cell lung cancer (NSCLC) cell lines as platform for cancer proteomics and biomarker discovery
Biochim. Biophys. Acta, May 2014; 1844: 950–959.
NOTE: Fast Green FCF staining and imaging used for normalization of array signals to total protein.
- Alshareeda , A.T., et al.
SUMOylation proteins in breast cancer.
Breast Cancer Res. Treat., Apr 2014; 144: 519–530.
- Leivonen, S-K., et al.
High-throughput screens identify microRNAs essential for HER2 positive breast cancer cell growth.
Mol. Oncol., Feb 2014; 8: 93–104.
- Puard, V., et al.
Semi-quantitative measurement of specific proteins in human cumulus cells using reverse phase protein array
Reprod. Biol. Endocrinol., Oct 2013; 11: 100.
- Alshareeda , A.T., et al.
Clinicopathological significance of KU70/KU80, a key DNA damage repair protein in breast cancer.
Breast Cancer Res. Treat., Jun 2013; 139: 301–310.
- Meyer, C., et al.
Transcript and protein profiling identifies signaling, growth arrest, apoptosis, and NF-κB survival signatures following GNRH receptor activation
Endocr. Relat. Cancer., Feb 2013; 20: 123–136.