Figure 1. Validation of siRNA knockdown in A431 cells by two-color quantitative Western Each siRNA target is detected in green. In red, tubulin was detected as used as a loading control to normalize for sample variation. Graph indicates % of target expression in siRNA-treated cells, relative to controls. In each blot, the marker is at the left, the negative control is in the center, and the siRNA is at the right.
Figure 2. Clear, detailed blot images of siRNA knockdown of ERK2 was performed in A431 cells. ERK2 detection is shown in green (primary antibody binds both ERK isoforms); tubulin loading control is shown in red. In this Western blot, the spatial resolution of ERK isoforms demonstrates that when ERK2 expression is lowered with siRNA treatment, ERK1 expression increases.
Quantitative Western blotting is an essential tool for every step in your RNAi studies:
The Odyssey® CLx Infrared Imaging System provides accurate digital protein data for your RNAi studies.