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TILLING

tilling fig 1

Figure 1

Rapid acquisition of genomic sequence data has elevated a new discipline, functional genomics, which focuses on determination of gene function. Reverse genetics methodologies are an important part of functional genomics.

Traditional reverse genetic methods, such as the use of transposons to "knock out" a specific gene, can accurately determine phenotype but require time consuming transgenic or sophisticated tissue culture methodologies. Such "knockout" methods are limiting because the entire gene is knocked out - the effects of partial loss of function of an active gene cannot be observed.

To overcome the limitations of knocking out an entire gene and to expand knowledge of active gene mutations, researchers from Fred Hutchinson Cancer Research Center developed a process for Targeting Induced Local Lesions In Genomes, or TILLING.

Elegantly simple, yet highly efficient, TILLING uses chemical mutagenesis to yield a traditional allelic series of point mutations for virtually all genes. The TILLING process is of particular value for essential genes where sublethal alleles are required for phenotypic analysis. The value of TILLING for genetic research is enhanced by its proven viability for a rapidly growing range of organisms.

  • Drosophila
  • Arabidopsis
  • Maize
  • Medicago truncatula
  • Zebrafish
  • and more...


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