Figure 1. Sagittal sections of mouse brain, immunostained for cannabinoid (CB1) and dopamine (D2) receptors to monitor changes in G protein-coupled receptor levels. The images are pseudo-colored overlays of the two staining patterns, with CB1 in red and D2 in green (dual-expression in yellow). Scale bar = 1 mm. Reprinted with permission from Kearn, CS. LI‑COR Biosciences application note (2004).
Image courtesy of Dr Christopher Kearn, University of Washington.
The Odyssey® Systems can image tissue sections stained with IRDye® antibodies or optical probes. The sections can be labeled with standard immunohistochemical protocols or in vivo as part of an animal imaging study. Sections are imaged at 21 µM resolution for macro-level analysis. Many sections can be imaged in a single scan for screening, so that valuable microscope time is used more efficiently.
See the Microscopy page for further information on using LICOR dyes for microscope applications.
Images courtesy of Dr Christopher Kearn, University of Washington.
1. Kearn, CS. Immunofluorescent mapping of cannibinoid CB1 and dopamine D2 receptors in the mouse brain LI-COR Biosciences application note (2004)