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Tissue Section Imaging

EMSA performed with IRDye 700 AP-1 oligos

Figure 1. Sagittal sections of mouse brain, immunostained for cannabinoid (CB1) and dopamine (D2) receptors to monitor changes in G protein-coupled receptor levels. The images are pseudo-colored overlays of the two staining patterns, with CB1 in red and D2 in green (dual-expression in yellow). Scale bar = 1 mm. Reprinted with permission from Kearn, CS. LI‑COR Biosciences application note (2004).

confocal microscopy

Image courtesy of Dr Christopher Kearn, University of Washington.

The Odyssey® Systems can image tissue sections stained with IRDye® antibodies or optical probes. The sections can be labeled with standard immunohistochemical protocols or in vivo as part of an animal imaging study. Sections are imaged at 21 µM resolution for macro-level analysis. Many sections can be imaged in a single scan for screening, so that valuable microscope time is used more efficiently.

See the Microscopy page for further information on using LICOR dyes for microscope applications.

Key advantages of using one of the Odyssey Imagers for tissue sections:

  • Quickly screens multiple sections prior to microscopic analysis.
  • Permits multiplexed detection of two different targets.
  • Allows quantification of staining.
  • Does not require restaining after in vivo imaging experiments with IRDye optical probes.
21 micron resolution of c-terminus cannabinoid receptor in the 700 nm channel on the Odyssey Infrared Imaging System. odyssey imager 3

Images courtesy of Dr Christopher Kearn, University of Washington.

1. Kearn, CS. Immunofluorescent mapping of cannibinoid CB1 and dopamine D2 receptors in the mouse brain LI-COR Biosciences application note (2004)

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