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Transporter Targeting

Nude mouse bearing a subcutaneous epidermoid (A431) carcinoma.

Figure 1. Nude mouse bearing a subcutaneous epidermoid (A431) carcinoma. 15 nmol of IRDye 800CW 2-DG was injected via tail vein 24h prior to imaging. Image captured with the Pearl® Imager with the pseudo-color representing the 800nm channel2.

Cell surface transporters can be used as targets for in vivo imaging by injection of agents that bind specifically to the transporter.

  • Tumors are often more metabolically active than surrounding normal cells, and may have an increased rate of glucose metabolism and cell surface glucose transporter activity1.
  • This property is widely exploited in the clinical setting for PET imaging with 18FDG.
  • The GLUT1 glucose transporter can be targeted with a fluorescently-labeled IRDye 2-deoxyglucose (2-DG) agent for longitudinal studies of tumor progression2.

Near-infrared dyes, such as IRDye fluorophores, and carefully optimized hardware are critical for high-performance optical imaging.

  • Near-infrared fluorophores exploit the spectral region where light absorption and scatter properties of tissue are most advantageous3. This enhances penetration depth (access of excitation light to the fluorophore) and escape of emitted fluorescence from the animal to reach the detector.
  • Laser illumination delivers very intense excitation light of the correct wavelength, generating the brightest possible signal from the fluorescent agent.
  • Intrinsic autofluorescence from animal tissue can mask the signal from optical probes. In the NIR spectral region, autofluorescence is dramatically lowered4,5.

Note: IRDye® 680LT dye products should not be used for small animal in vivo imaging or In-Cell Western™ Assays. The higher level of fluorescent intensity creates high background making it unfavorable for use in these applications. We recommend using IRDye 680RD dye products for these applications.

  • 1. Gambhir, SS et al. Journal of Nuclear Medicine 42: 1S-93S (2001)
  • 2. Kovar, J et al. Analytical Biochemistry 384: 254-262 (2009)
  • 3. Hawrysz, DJ and Sevick-Muraca, EM. Neoplasia 2(5): 388-417 (2000)
  • 4. Frangioni, JV. Curr Opin Chem Biol. 7(5): 626-34 (2003)
  • 5. Adams, KE, et al. J Biomed Opt. 12(2): 024017 (2007)
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