The In‑Cell Western™ Assay: Fast, Accurate Measurement of Protein Levels for Quantitative Immunofluorescence
On overview of how the In-Cell Western Assay works, optimization tips, and several examples of the In-Cell Western in action: signal transduction, comparison to Western blotting and other assays, utility of increased throughput, and analysis of cell surface proteins.
Date: November 1, 2008
Amy Geschwender, Ph.D.
Advance video to...
- Introduction - 0:00
- Why Near-Infrared Fluorescence? - 1:58
- In-Cell Western Assay - 3:06
- How does the In-Cell Western Assay Work? - 4:39
- Imaging with the Odyssey and Data Analysis - 6:21
- Advantages of ICW Assays - 7:35
- Streamlined Workflow - 10:52
- Simple, Affordable Alternative to Flow Cytometry or HCS - 12:28
- How do you Optimize the Assay? - 14:00
- How do you correct for Well-to-Well Variation? - 19:59
- Signal Transduction: EGFR Signaling - 24:29
- Comparison to Western Blotting and Other Assays - 25:54
- Why is Increased Throughput Useful? - 33:30
- Can you Analyze Cell Surface Proteins? - 39:02
- LI-COR Infrared Imaging Systems and Reagents - 41:04
- Summary & Acknowledgements - 41:58
- Q&A - 43:35
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