Normalization Methods for Quantitative Analysis of Western Blots
Get practical, expert advice on how to normalize. Learn how to use different normalization methods to generate high-quality data suitable for quantitative comparisons. By understanding how to remove sources of error and reduce variability, you can ensure the integrity of your data.
Normalization methods include:
- Using internal reference proteins, such as housekeeping proteins or pan/phospho analysis
- Using a total protein control
LI-COR imaging systems support all methods of normalization. Learn more about normalization methods and strategies for determining the best method for your experimental conditions.
October 11, 2016
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