Western Blot Normalization: What You Need To Know
Western blotting is packed with sources of variability, which affects data accuracy and reproducibility. Learn what normalization is and why it is critical for quantitative Western blot analysis. In this webinar, Dr Geschwender explores important considerations for different normalization methods.
Normalization methods include:
- Using internal reference proteins, such as housekeeping proteins or pan/phospho analysis
- Using a total protein control
LI-COR imaging systems support all methods of normalization. Learn more about normalization methods and strategies for determining the best method for your experimental conditions.
September 20, 2016
Amy Geschwender, PhD
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