Grid Background Group

The following options are available in the Background group.

• Background list - Choose a background subtraction method from the Background list.
• Average - Uses space surrounding a spot as background.
• Median - Uses space surrounding a spot as background.
• User-Defined - A spot or spots must be designated as background.
• None - Unless data are going to be exported for analysis using a third-party software, do not choose None.
• Assign - When the User-Defined background method is chosen, this button is used to assign a spot to be used for background calculation.
• Unassign - Removes a User-Defined background spot from the background calculation.