LI‑COR® Acquisition Software Quick Start

This guide will get you started using LI‑COR Acquisition Software to acquire images with LI‑COR imagers. The Empiria Studio® Software Quick Start Guide covers getting started with image analysis.

Download and Install LI‑COR Acquisition Software

Ensure you have the correct permissions to install software on the computer you want to use, and ensure that your computer has the required specifications. LI‑COR Acquisition is not available for macOS.

  1. Download LI‑COR Acquisition from

  2. Click the downloaded installer file.

  3. Follow the steps in the installation wizard to complete installation.

Check Computer's Sleep Settings

If your computer goes to sleep during image acquisition, the image acquisition may not complete as expected.

Work with the appropriate people from your organization to ensure that the computer does not go to sleep during image acquisition. The most reliable way to ensure the computer does not go to sleep is to ensure your computer's settings prevent it from going to sleep automatically. To change the settings, go to Start > Settings > System > Power & sleep or click this link: Power & sleep settings.

Start LI‑COR Acquisition

Start LI‑COR Acquisition Software by double-clicking the LI‑COR Acquisition icon on the Desktop.

Create an Image Data Folder

You will be prompted to create an Image Data Folder A folder on your computer's hard drive where internal LI‑COR Acquisition data will be saved. where LI‑COR Acquisition can store internal software data. Follow guidelines in the software.

Do not put your Image Data Folder on a network drive or in a folder that automatically syncs with the cloud. Software can be slow when accessing network folders, and data can be lost if multiple people access the data simultaneously.

  1. Open the Windows File Explorer Windows file explorer icon.

  2. Create an overall folder to hold Image Folders (e.g., "LI-COR Acquisition Images"), then create an Image Data Folder within the overall folder (e.g., "Image Data Folder 1").

    LI-COR Acquisition Software data folder structure
    Example Image Folder Structure. You and your research group must choose a folder structure that works for you.
  3. In LI‑COR Acquisition, select the Image Data Folder (e.g., "Image Data Folder 1").

Acquire Your First Image

  1. On the Home page , select the username that you want to use, or click New Username to add a new username.

    Your LI‑COR Acquisition username helps you track your acquired images and is not associated with your LI‑COR account.

  2. Select the Image Data Folder that you want to use, or click Add Data Folder to add a new Image Data Folder.

  3. Click Scan .

  4. Select the imager you want to use, and click Connect.

  5. Choose an image acquisition workflow.

    • To use preset settings that are generally recommended as a starting point, click the name of the assay you want to image.

    • To use custom settings, click Custom. You may use custom settings, for example, if you are optimizing imaging settings for your particular assay.

Export Acquisitions and Images

  1. On the Home page, click Image Gallery Image gallery icon.

  2. Select one Acquisition or multiple Acquisitions to export.

    LI-COR Acquisition multiple image gallery selections
    Use SHIFT + Click or CTRL + Click to select multiple Acquisitions.
  3. From the menu above the Image Gallery, click an export option.

    Export to Analyze: Export the Acquisitions to a file that can be brought into Empiria Studio for analysis.

    Export to Publish or Present: Export image(s) to use in a presentation or publication. These image formats should not be used for analysis.

    Export to Image Report: Export image Acquisition(s) and important information about the image Acquisition(s) to a PDF.