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The C‑DiGit® is the closest I’ve found so far to replacing film,” says Dr. Morano, “and it is the first thing I’ve seen come around that has really encouraged me to say we can get rid of the film developer.

Dr. Kevin Morano
Professor, Department of Microbiology and Molecular Genetics
The University of Texas Graduate School of Biomedical Sciences at Houston

Dr. Stanislav Kholmanskikh

Students in Dr. Morano’s lab analyzing Western Blotting results using the C-DiGit® Blot Scanner

Dr. Kevin Morano is a Professor at the University of Texas Medical School in Houston, in the Department of Microbiology and Molecular Genetics. Dr. Morano and his graduate students, part of the University of Texas Graduate School of Biomedical Sciences, study the transcriptional response to heat shock in baker’s yeast, Saccharomyces cerevisiae, and how protein chaperones, specifically the Hsp70, Hsp90, and HSP110 groups, function within cells.

Each of the students in the lab is investigating different aspects of these questions using various techniques. One of the most common techniques is evaluating and quantifying protein expression using the Western blot. The lab tried many imagers and imaging techniques to obtain the quality Western blot data they were looking for. According to Dr. Morano the lab has been relying on film as their go-to method for capturing Western blotting data for over a decade.

A Hassle-Free Replacement for Film

There are some issues with that solution, though, explains Jennifer Abrams. "You can’t really use film to do quantification, because [film response to light] is not linear." She adds, "For publications in our field now, you have to have quantification."

That was the reason that Dr. Morano decided to look into the C‑DiGit® Blot Scanner. "The C‑DiGit is the closest I’ve found so far to replacing film," says Dr. Morano, "and it is the first thing I’ve seen come around that has really encouraged me to say we can get rid of the film developer. It’s reducing our film usage substantially."

Obtaining Western blot data is important to the Morano lab, and sometimes the department developer is down for a day or two. Graduate student Veronica Garcia exclaimed, "We can’t go a day or two without having a way of visualizing our Westerns." This is a hindrance, because the shared developer requires a lot of maintenance, and general upkeep is expensive — especially compared to the C‑DiGit Blot Scanner. Dr. Morano adds "It can be argued that there are more sensitive chemi imagers out there, but certainly nothing this good for under $5,000."

Reaping the Benefits of the C-DiGit Scanner

I think my students pretty much fight to be able to use it.

Other advantages for the lab include time and cost savings. "I think the C‑DiGit does a really good job of getting rid of all that extra hassle and expense," says Garcia. Dr. Morano adds, "I’ve actually been touting this machine to anybody that will listen to me... I think it’s a great resource for the lab or maybe a couple of labs to share if they do a lot of Westerns."

Thankfully, sharing the C‑DiGit Blot Scanner works well for Dr. Morano and his students, because when the departmental developer and chemiluminescent imager are down, other people stop by to use it. "It has worked well for all of them," says Garcia. And, says Morano, "I think my students pretty much fight to be able to use it."

We are proud to call Dr. Kevin Morano and his graduate students C‑DiGit Blot Scanner experts.



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