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Simultaneous detection and quantification of two different antigens is the optimal and fastest way to obtain results.

Manuela Oana Gustafsson
Associate Researcher
Karolinska Institute's Department of Laboratory Medicine in the Molecular Cell Biology and Gene Therapy Science group

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Manuela Oana Gustafsson is an Associate Researcher at Karolinska Institute's Department of Laboratory Medicine in the Molecular Cell Biology and Gene Therapy Science group. The group leader there, C. I. Edvard Smith, discovered a mutation in Bruton's Tyrosin Kinase (Btk) in his research that causes X-linked human immunodeficiency disease, Agammaglobulinemia (XLA). Btk has become the target of Smith's lab's research, and is Gustafsson's main research focus. She works to identify and characterize new Btk binding partners and study how their interactions relate to disease development and progression.

Manuela Gustafsson

Enabling Multiplex Western Blotting

Gustafsson has worked with the Odyssey® Infrared Imager extensively for about a year. In fact, it has been instrumental in moving Gustafsson's research forward. She has grown to appreciate its usefulness. The Odyssey Infrared Imager's dual-color multiplexing capabilities have been particularly beneficial in her phosphorylation studies. "I am so happy with this method. Simultaneous detection and quantification of two different antigens is the optimal and fastest way to obtain results."

Gustafsson's lab has had the Odyssey Infrared Imager for nearly two years. The best part, she explains, is that the Odyssey Infrared Imaging System and Image Studio Software are easy and user-friendly to use. "Everybody has a separate user account in the Cell Signaling part of the lab, which does mainly infrared Western blotting and In‑Cell Western™ Assays. You get accurate digital data, all in one place and it is secured from unauthorized access."

A Sustainable and Reliable Technology

Gustafsson appreciates that infrared Western blotting techniques are much more convenient and meet the environmental standards of the Karolinska Institute much better than the chemiluminescent methods she and her group mates had been using in the past. "With a chemiluminescent blot you have to run and hurry to develop a film. Hopefully no one is in the darkroom when you get there."

I am very happy with the technical support that we have from LI-COR.

LI-COR Biosciences' sales representatives and support teams also play a large role in Gustafsson's success. She explains, "I am very happy with the technical support that we have." LI‑COR has technical support representatives spread across various territories. Describing her experience, Gustafsson adds, "The Technical Sales Consultant here in Sweden is a great person. Whenever I need advice, I can call her and she will help."

Gustafsson recently published a paper with Anthony Pawson on the regulation of nucleocytoplasmic shuttling of Btk, and has another paper that has been accepted for publication soon. Her success, and trust in LI‑COR technology and support, are just some of the reasons we are proud to call Manuela Gustafsson an Odyssey Expert.

Publications

Publications resulting from work on the Odyssey

  1. Gustafsson MO, Hussain A, Mohammad DK, Mohamed AJ, Nguyen V, Metalnikov P, Colwill K, Pawson T, Smith CIE, Nore BF. Regulation of nucleocytoplasmic shuttling of Bruton's tyrosine kinase (Btk) through a SH3-dependent interaction with ankyrin repeat domain 54 (ANKRD54) Mol Cell Biol 32(13): 2440-53. Epub 2012 Apr 23
  2. Hussain A, Mohammad DK, Gustafsson MO, Uslu M, Hamsay A, Nore BF, Mohamed AJ and Smith CI. Signaling of the ITK (Interleukin 2-inducible T Cell Kinase)-SYK (Spleen Tyrosine Kinase) Fusion Kinase Is Dependent on Adapter SLP-76 and on the Adapter Function of the Kinases SYK and ZAP70. J Biol Chem. 288(10): 7338-50. 2013 Mar 8 doi: 10.1074/jbc.M112.374967. Epub 2013 Jan 4


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