The ability to quantitate those bands is just amazing!
Dr. Wendy L. Picking
Department of Microbiology and Molecular Genetics at Oklahoma State University
Dr. Wendy L. Picking, Associate Professor in the Department of Microbiology and Molecular Genetics at Oklahoma State University, studies enteric pathogenic bacteria, with a specific focus on Shigella flexneri.
Targeting the Secretion System of Pathogenic Bateria
Her work with Shigella reaches far beyond an understanding of this pathogen, but to other pathogenic organisms with type III secretion systems as well, such as E. coli, Salmonella, Yersinia, Pseudomonas , and Burkholderia. Her lab’s research on the type III secretion system (TTSS) has the ultimate goal of preventing infection by these pathogens via drug therapy or vaccines.
Dr. Picking’s lab collaborates closely to the adjacent lab, that of her husband and Department Head, Dr. Bill Picking. Combined, the two labs have 10-15 personnel including post doctoral researchers, graduate students and undergraduate students contributing to this research.
The Shigella bacterium is not often seen in the U.S., but outbreaks are seen in daycares and adult centers. Shigella is a problem in third world countries where water isn’t purified or in the case of disaster, such as military intervention, or after some natural events, such as after the recent Haiti earthquake. Shigella causes shigellosis, which is an important cause of childhood mortality throughout the developing world. Other pathogens with type III secretions systems, such as Salmonella and E. coli are a problem in the U.S., however. Pseudomonas is an important pathogen in burn victims and people with cystic fibrosis. Burkholderia is deadly to those with cystic fibrosis.
The type III secretion system is a molecular needle and syringe that is embedded in the membranes of many gram-negative pathogens. Dr. Picking’s lab is currently investigating the proteins that localize to the tip of that needle. They have found that invasion plasmid antigen D (IpaD) resides at the tip and have found ways to localize IpaB (a translocator protein), another key virulent, and finally IpaC. They are working towards understanding the type III secretion systems because these systems are common virulent mechanisms in gram-negative bacteria to transport effector proteins from the cytoplasm of host cells towards the benefit of the bacterium, and in Shigella’s case, it causes phagocytosis of the Shigella, and Salmonella as well. Dr. Picking believes that through the understanding of the type III secretion system, new antimicrobial drugs and vaccine therapies can be developed.
Helping Researchers Generate Quantifiable and Reliable Data
Dr. Picking described that not only is quantification good for deciding in which direction to take her research, but important for publication purposes as well. In referring to her manuscript “Lipsomes Recruit IpaC to the Shigella flexneri Type III Secretion Apparatus Needle as a Final Step in Secretion Induction” (Epler et al.), she explains:
If you quantify it and put it on a graph, you see that there are substantial differences between those bands.
“Based on the image of the Western blot, you don’t see a great deal of difference between some of the bands; but, if you quantify it and put it on a graph, you see that there are substantial differences between those bands. So that is important to us to be able to quantify that amount. And it was especially important for that particular experiment because that experiment is not a trivial experiment to do you don’t get too many chances to do that experiment right very often.”
Dr. Picking acknowledges that the results in this manuscript couldn’t have been attained without the Odyssey. Chemiluminescence cannot give legitimate quantification, and since she approaches her research area as a biochemist, legitimate quantification is very important.
Dr. Picking’s appreciation of the Odyssey’s capabilities, her commitment to her research, and the publications that have come as a result, are just some of the reasons she is considered one of LI-COR’s Odyssey® Experts.
For more information about Dr. Picking, visit Dr. Wendy Picking’s faculty profile at Oklahoma State University.
Publications resulting from work on the Odyssey
- Marianela Espina, Andrew J. Olive, Roma Kenjale, David S. Moore, S. Fernando Ausar, Robert W. Kaminski, Edwin V. Oaks, C. Russell Middaugh, William D. Picking, and Wendy L. Picking. IpaD Localizes to the Tip of the Type III Secretion System Needle of Shigella flexneri. Infect. Immun., Aug 2006; 74: 4391 - 4400.
- Chelsea R. Epler, Nicholas E. Dickenson, Andrew J. Olive, Wendy L. Picking, and William D. Picking. Liposomes Recruit IpaC to the Shigella flexneri Type III Secretion Apparatus Needle as a Final Step in Secretion Induction. Infect. Immun., Jul 2009; 77: 2754 - 2761.
- Andrew J. Olive, Roma Kenjale, Marianela Espina, David S. Moore, Wendy L. Picking, and William D. Picking. Bile Salts Stimulate Recruitment of IpaB to the Shigella flexneri Surface, Where It Colocalizes with IpaD at the Tip of the Type III Secretion Needle. Infect. Immun., May 2007; 75: 2626 - 2629.
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