Adapt chemiluminescent Westerns to NIR fluorescence with Chemi-IR™
Chemi-IR™ kits from LI-COR Biosciences adapt familiar chemiluminescent Western methods to quantitative, near-infrared fluorescent detection
July 9, 2009, Lincoln, NE: LI-COR® Biosciences announces the introduction of Chemi-IR™ Detection Kits – a bridging technology to help researchers convert from conventional chemiluminescent Western blotting to quantitative detection with near-infrared (NIR) fluorescence. "These products make familiar chemiluminescent methods quantitative," says Tony Endozo, LI-COR product manager. Chemi-IR kits include an affinity-purified rabbit anti-HRP antibody conjugated with the IRDye 800CW fluorophore and antibody diluents that are optimized for use with LI-COR Biosciences Odyssey® Infrared Imaging and Aerius® Automated Infrared Imaging Systems. These instruments have set a new standard for quantitative Western blotting, In-Cell Western™ and On-Cell Western cell-based assays, and many other applications.
With Chemi-IR detection, researchers can perform NIR fluorescent detection on Western blots that were incubated with standard HRP-conjugated secondary antibody. "Bands that once required long film exposures can now be quickly visualized and quantified," says Endozo. The IRDye fluorescent method typically yields equivalent or higher sensitivity than chemiluminescence, with several key advantages: linear and quantitative fluorescent signal, elimination of film and darkroom, and lower cost. The linear dynamic range is wider, so strong and weak signals are clearly detected on the same image without band "blow-out". Chemi-IR is compatible with many commercial HRP conjugates and chemiluminescent substrates.
About LI-COR Biosciences
LI-COR Biosciences is a leading manufacturer of near-infrared imaging platforms, analysis software, and LI-COR IRDye® infrared dye reagents for quantitative Western Blotting, small animal imaging, and DNA fragment analysis. The company also offers accessories and reagents for other detection types.
LI-COR pioneered the development of near-infrared fluorescence detection systems for DNA sequencing and today provides systems for drug discovery, protein research, small animal imaging, and undergraduate training. LI-COR instruments for photosynthesis, carbon dioxide analysis and light measurement are recognized worldwide for standard-setting innovation in plant science research and environmental monitoring. Founded in 1971, the privately held company is based in Lincoln, Nebraska, with subsidiaries in Germany and the United Kingdom. LI-COR systems are used in over 100 countries and are supported by a global network of distributors.