Odyssey XF Dual-Mode Imaging System for Western Blots and DNA Gel Imaging
Odyssey XF

Odyssey® XF
Imaging System

Get clearer answers with accurate, replicable Western blots and DNA gels.

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Odyssey XF
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See more with additional channels and clearer images

Image your near-infrared (NIR) and enhanced chemiluminescent (ECL) Western blots, detect multiple targets on the same blot with two channel multiplex fluorescence, and document DNA gels all on the same imager, without juggling multiple instruments.

See the full story in a single image

Increase the consistency and accuracy of your Western blot results by capturing a single image, without comparing multiple exposures or changing camera settings. The patented FieldBrite™ XT2 optics that power the Odyssey XF allow you to get the best image every time.

Odyssey XF Western Blot Image Sequence 1 Odyssey XF Western Blot Image Sequence 2 Odyssey XF Western Blot Image Sequence 3 Odyssey XF Western Blot Image Sequence 4 Odyssey XF Western Blot Image Sequence 5 Odyssey XF Western Blot Image Sequence 6 Odyssey XF Western Blot Image Sequence 7 Odyssey XF Western Blot Image Sequence 8 Odyssey XF Western Blot Image Sequence 9 Odyssey XF Western Blot Image Sequence 10 Odyssey XF Western Blot Image Sequence 11 Odyssey XF Western Blot Image Sequence 12 Odyssey XF Western Blot Image Sequence 13 Odyssey XF Western Blot Image Sequence 14 Odyssey XF Western Blot Image Sequence 15 Odyssey XF Western Blot Image Sequence 16 Odyssey XF Western Blot Image Sequence 17 Odyssey XF Western Blot Image Sequence 18

One image file contains the full range of your data. Choose the best image display for further analysis, based on your protein of interest. Changing the image display settings never alters your raw data or signal intensities.

See more with more channels

Detect your chemiluminescent or NIR Western blots and document DNA gels on the same imager without juggling multiple instruments. Document your agarose and PAGE gels stained with Ethidium Bromide, SYBR® Safe, SYBR Green I, and many other widely used DNA stains.

700nm and 800nm Western Blot
700nm Western Blot
800nm Western Blot
Overlay 700 nm 800 nm
Multiplex to detect two different protein targets in each sample lane. Use secondary antibodies labeled with spectrally-distinct NIR fluorescent dyes to get more data from your blot. View, adjust, and analyze your results as a merged image, or as separate 700 nm and 800 nm channel images in pseudo-color or grayscale.

Do more with consistent, accurate digital imaging

Capture uniform, low-background images that are not subjected to image correction. Because post-capture image manipulation is not required, the Odyssey XF provides superior data reliability.

Get the highest quality images without artificial software enhancements

Other digital imagers have been known to modify images after capture to correct for non-uniform image brightness or to artificially improve the exposure. Other imagers manipulate images post-capture by:

  • Binning: Some CCD imagers combine groups of pixels to improve signal-to-noise ratio, which reduces image resolution.
  • Flat fielding: Other CCD imagers need to correct for non-uniform illumination caused by the lens, filter, or other optical components. These imagers alter image data through a software algorithm called “flat fielding” to compensate for non-uniformity.
  • Image stacking: Some imagers acquire many images to get the nicest looking data. These imagers will select the best image from a “stack” of images.

The patented optics of the Odyssey XF deliver uniform, low background images every time with no post-capture image manipulation. With the Odyssey XF, you can be confident you have the most accurate, replicable data.

binning data loss
binning data loss
binning data loss
binning data loss

Other CCD imagers artificially improve signal-to-noise (SNR), but sacrifice image resolution.“Binning” is a software algorithm that combines groups of pixels to create an image with improved SNR, but reduced image resolution. Because the Odyssey XF is designed to collect low-background images, binning is not necessary, and faint bands can be detected at the CCD’s full resolution.

Discover more with richer, more complete data

Get consistent, accurate digital images so you can analyze your data right away and plan your next experiment.

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See more without changing settings or sacrificing data integrity

Capture the true detail and complexity of your data. With 6 logs of linear dynamic range, your results are more reliable. Near-infrared fluorescence delivers consistent signals that aren’t affected by timing.

Capture all of your Western blot data (weak and strong) in clear, low-background images every time without adjusting camera settings. To see the whole picture, you need enough capacity (dynamic range) to consistently document your strongest bands. This allows for accurate comparison within and between labs and experiments.

 
Film has a shallow data capacity and cannot accurately record strong signals. Once that capacity is reached, all additional signal (read: your data) is simply lost. Many CCD imagers are also limited by data capacity and image saturation.
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Imaging applications

The versatile imaging technology of the Odyssey XF Imager is optimized for a variety of applications, helping propel the Odyssey imager family to over 25,000 peer-reviewed publications.

The Odyssey XF Imager is optimized for a variety of applications, including:

  • Near-Infrared Fluorescence and Chemiluminescence Western Blots
  • Nucleic Acid Gel Documentation
  • Coomassie Protein Gel Documentation
  • Near-Infrared Fluorescence and Chemiluminescence Protein Arrays

“Instead of getting two or three different instruments for imaging Western blots, agarose gels, SDS-PAGE gels, and other assays, we purchased a single instrument that can be used for all of these applications.”

Rick Page, Miami University
Quantitative NIR Western Blot

Quantitative NIR Western Blot

Chemiluminescent Western Blot

Chemiluminescent Western Blot

Nucleic Acid Gel Documentation

Nucleic Acid Gel Documentation

Coomassie Protein Gel Documentation

Coomassie Protein Gel Documentation

Acquisition and analysis software for the Odyssey XF

Pair your Odyssey XF Imager with Empiria Studio® Software for streamlined image acquisition, organization, and analysis of your Western blots.

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LI-COR Acquisition Software

LI-COR Acquisition Software

LI-COR Acquisition Software is used to acquire, organize, and adjust images from your Odyssey XF Imager. Workflows guide you through the necessary steps using identical settings based on assay type to minimize sources of variability. Images are organized by assay and can be adjusted then exported for publication or analysis in Empiria Studio Software.

Empiria Studio

Expert Western blot analysis made simple with Empiria Studio Software

Designed for the analysis of near-infrared Western blot images acquired on an Odyssey imager, Empiria Studio is the only Western blot analysis software that guides you all the way from validation to statistical analysis for reliable results. Streamlined workflows minimize user-to-user variation, compute statistical values, and provide extensive analysis options.

Learn More about Empiria Studio

Odyssey XF

See the Odyssey XF in action

Get the most accurate results from more than 6 logs of linear dynamic range for both NIR fluorescent and ECL Western blots.

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* Remote demonstrations are available.

Looking to include tissue sections and plate-based assays?

With the Odyssey Duo, bundle an Odyssey DLx and an Odyssey XF together and save on the cost of purchasing each imager separately. Equip your lab with access to two near-infrared fluorescent Western blot imagers, a chemiluminescent Western blot imager, and an extended list of other applications.

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