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Compatibility

The MPX is compatible with all common Western blotting procedures that generate a nitrocellulose or PVDF blot of 7 cm x 8.5 cm. It can be utilized with all standard chemiluminescence, colorimetric, and infrared protocols. The channel ports are spaced to accommodate most common multichannel pipettes.

MPX vs Standard Western Blot Cost Comparison Using Near-Infrared Detection

Reagents IR Detection
2-targets
(15-well gel)
Chemiluminescence
(15-well gel)
24-well MPX
IR Detection
1st -target 2nd-target
Blocking Buffer $7.80 $7.80 $7.80 $5.20
Primary Antibody
ß-Tubulin (LI-COR, 926-4211)
$239 (100µL)
ERK 1 (Santa Cruz SC-94)
$229 (200 µg/mL,200µL)
$23.56
$18.32
$23.56
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$18.32
$4.71
$1.04
Secondary Antibody
(Recommended Dilutions: 1:15,000 for IR, 1:25,000 for Chemi, 1:5,000 for MPX)
$0.82 $0.09 $0.09 $0.08
Chemiluminescent Substrate
(0.1 ml/cm2)
$0.00 $12.20 $12.20 $0.00
Film (2 sheets/blot) $0.00 $7.71 $7.71 $0.00
Protein Markers
(Recommended Amount: 1µL for IR; 10 µL for Chemi)
$0.22 $2.26 $2.26 $0.22
$ per Data Point $1.69 $3.51 $3.23 $0.23
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