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Increase your productivity by eliminating typical darkroom hassles – like taking multiple exposures or waiting in line. Instead, get the full story in minutes with a single scan.
Find out what’s really happening in highly abundant samples by revealing key differences in strong signals. See both strong and faint bands together in a single digital file.
Leave blurred bands and saturation behind. Capture true detail and complexity from every Western blot, due to advancements in optical design and engineered efficiency.
Eliminate typical darkroom hassles, like taking multiple film exposures, waiting in line, and processor jams. Your digital image is ready in as little as 6 minutes – faster and easier than walking to the darkroom, exposing and developing film, and scanning the film to create a digital image.
This personal-size imager is about the size of a lab notebook. It fits where you need it, when you need it. While you’re saving benchtop space, scrap your darkroom as well. What else could you do with that darkroom space?
Lab costs can add up quickly, especially with darkroom immunoblotting. Consider processor and developer maintenance, silver recycling fees, hazardous photographic chemicals, and the cost of film itself. Upgrade your Western blots to digital detection, and stretch your grants farther.
Detection is faster and easier than ever before. Unlike some imagers, there’s no need to adjust exposure settings or capture multiple images. The simplicity of this compact machine means every user experiences consistent data capture. The result: you get high quality images the first time, every time.Order Now
“With limited research funding, identifying poor quality antibodies and poor Western blotting techniques will save money, save researchers time and improve the quality of the results.”
Gilda et al (2015) PLoS ONE. 10(8).
Reliable analysis of different signal intensities is possible, when you bypass the limitations of film detection. Normally, multiple exposures are necessary to view faint and strong bands, and they’re usually on separate pieces of film. With the C-DiGit Blot Scanner, you can capture strong chemiluminescent signals without sacrificing detection of faint bands.
“…Digitally acquired images provide the more accurate representation of band intensity and its relationship to sample abundance.”
Janes (2015) Science Signaling. 8(371).
Get a more secure alternative to keeping track of multiple pieces of film. Direct digital detection enhances your ability to analyze immediately, as well as refer back to previous experiments. You’ll have access to all the data you need today – and months from now, when that blot is critical for your next paper or grant.
Digital imaging is necessary to show what’s really happening in highly abundant samples. Film only shows you part of the picture, because it has a limited data capacity. When you capture strong signals, you need a sufficiently wide linear dynamic range to analyze your full range of data.Order Now
Strong signals blur on film and can overlap adjacent bands. Light scatter is the primary cause of this “blowout,” and is greatly reduced by the advanced imaging optics of the C-DiGit Blot Scanner.
Strong signals are also typically under-represented on film, and in quantitative analysis. Strong bands can easily exceed the darkening capacity of the film, creating saturated signals. When saturation occurs, band intensities appear different, but relative signal intensity plateaus. It’s important to note that film signal response begins to plateau even before saturation is reached.
Capture a complete data set that has vastly improved data quality. One major advantage is the cumulative integration of multiple time points of your blot. Analyze data with true detail and complexity, instead of several separate “snapshots” of a quickly changing enzymatic reaction.
Choosing the “best” exposure qualitatively out of many images is subjective to each end user. A fully customizable protocol increases the chances of variability creeping into your experimental results. Choose an imager that will enhance reproducibility, rather than introduce more variability.
“Any device used for digitizing must have an ample, linear and known dynamic range.”
Gassmann et al (2009) Electrophoresis. 30:1845-1855.
Take back your benchtop and stretch your funding farther with this compact, affordable scanner. One digital file contains all your ECL data. See key differences in strong signals, and get consistent data quality without “blowout” or saturation.
“Others in our lab and especially first-timers, who have never done blots before, like it because it is streamlined and straightforward.”
Lisa Keyes, University of Florida
Get your money back within 30 days if the
C-DiGit isn’t right for your lab.