Discover More with C-DiGit Western Blot Imaging Technology.
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The C-DiGit® Chemiluminescent Western Blot Scanner

Read about making your experiments simpler and more efficient in Digital Film for Westerns.


Comparison of film and the C-DiGit® Blot Scanner

Read about two critical variables that affect accuracy in Western blot analysis.


Discover More with Advanced Optics

Read about the detection system that was previously considered impossible in 'Imaging Chemiluminescence by Scanning.'


C-DiGit® Blot Scanner

How It Works

 

Technology that enables you to do more, see more, and discover more

Do More with Direct Digital Capture

After a single scan, your C-DiGit image file is a complete picture of all the data for each Western blot. Unlike film, there’s no need for multiple exposures to view strong bands and faint bands, or to make sure you’ve captured all the information you might need in the future. ECL requires you to move fast to capture signals before they’re gone. Skip the darkroom with direct digital capture, and get your results immediately – ready for analysis and publication.

Read more about how to increase your productivity in this white paper: Digital Film for Westerns: The C-DiGit® Chemiluminescent Western Blot Scanner

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One C-DiGit scan represents the full range of ECL data for that blot.
One C-DiGit scan represents the full range of ECL data for that blot. Choose the best display for further analysis, based on your protein of interest. Changing the display never alters your raw data or signal intensities.

See More with Accurate Detection

C-DiGit signals are proportional to light response, contributing to a wide linear dynamic range. Conversely, the response of film to light is governed by the reciprocity law: film response is supposed to be proportional to light intensity and duration of exposure.

When light intensity is very low, film response is lower than expected due to the photochemistry of creating a latent image. This causes faint signals to drop off abruptly on film, where a gradual decrease is expected. At the other end of film response, very strong signals exhibit high-intensity reciprocity failure. When this happens, film response plateaus and can no longer accurately record signals.

Read more about how you get accurate signal response with C-DiGit detection in this white paper: Comparison of film and the C-DiGit® Blot Scanner

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C-DiGit imaging is proportional to light response, showing gradual contrast in signal intensity.

C-DiGit imaging is proportional to light response, showing gradual contrast in signal intensity. Strong signals “blowout” on film (overlap adjacent bands), and film response plateaus even before it completely saturates (the darkening limit of film is exceeded). Faint signals’ intensities also drop off abruptly on film. With C-DiGit imaging, strong bands slowly increase in signal intensity (2.5 µg – 10 µg). In addition, gradual decreases for faint bands (0.625 µg – 2.5 µg) are clearly shown.

Discover More with Advanced Optics

The advanced optical design of the C-DiGit combines the advantages of film and CCD imaging. Like film, the C-DiGit has a short working distance for efficient signal capture, and it also has an immediate digital output (similar to CCD imagers). Choose the scan sensitivity you need: “standard” for strong signals (6 minute scan), or “high” for blots with less intense signals (12 minute scan).

Short scan times produce less dark noise – without requiring expensive sensors or supercooling. These short scan times are possible with fast line-reads that quickly capture data across the entire blot.

Read more about the advanced technology of the C-DiGit in this white paper: Imaging Chemiluminescence by Scanning.

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Get fast signal capture from advanced optical design and engineered efficiency.

Get fast signal capture from advanced optical design and engineered efficiency. The short working distance (imaging very close to the blot surface) of the C-DiGit enables very efficient light collection. Light is captured close to the blot surface, preventing photon loss. This boosts sensitivity without needing expensive sensors or supercooling of the instrument.

Who's Using It?

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