Get Consistent, Reliable Data with Digital ECL Imaging
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Get Consistent, Reliable Data

With Reproducible Digital Imaging

Spending Too Much Time in the Darkroom?

Many steps of immunoblotting create unpredictable variability. For better reproducibility, eliminate darkroom hassles with consistent digital detection.

Having Trouble Finding the Right Exposure?

Detect strong and faint bands in a single image the first time, every time. Capture true detail and complexity when you get all the data in one image.

Repeating Your Experiment, but Getting Different Results?

Analyze subtle changes accurately with a deeper data capacity. Get complete, reproducible data with advanced digital imaging.

Work at Your Bench, Not in the Darkroom

Get results quickly

Save time and minimize the number of manual steps that can introduce variability. Focus on what’s really important – your research – by eliminating broken developers, processor jams, and waiting in line. Capture the full range of ECL data in just one image, instead of piecing together multiple film exposures.

 

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See All the Data in One Image

View complexity instead of snapshots

Detection is faster and easier than ever before. Digital imaging removes user subjectivity from adjusting exposure settings or taking multiple images. You’ll capture consistent data with accurate, streamlined digital imaging. The result: a high quality image the first time, every time.

“…The range varies with the exposure time of the blot to film. The use of film is also a drawback as it is difficult to detect saturation of the signal on the film.”

Bond et al. Biol. Proced. 10(1):20-28 (2007).

How are Western blot results misrepresented by film and photochemistry?

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You're one step closer to understanding why using film affects the accuracy of your densitometry results.

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Capture Complete, Reproducible Data

Image strong bands without saturation

Digital imaging shows your strong signals as they really are. Film only shows you part of the picture, because data are lost when images become saturated. To see the whole picture, you need enough capacity (dynamic range) to consistently document your strongest bands. See all the data you’ve been missing with film – without sacrificing sensitivity.

“The ease at which an x-ray film becomes saturated with signal gives investigators a false sense that film is more sensitive than digital imagers.”

Ghosh et al. The necessity of and strategies for improving confidence in the accuracy of western blots. Expert Rev. Proteomics. 11(5):549-60 (2014).

 

Go Digital

Leave darkroom uncertainty behind, and get the consistency you need with digital detection. Devote your funding, time, and effort to something more productive than the darkroom.

“…There are a lot of variables in performing experiments and in collecting and analyzing data. Seemingly insignificant changes in execution or analysis can have profound impacts on results.”

Drubin, D. Great science inspires us to tackle the issue of data reproducibility. Mol. Biol. Cell. 26: 3679-3680 (2015).

Take Your Research Further

You ask big questions, and your detection method should be able to handle all the answers. Confidently analyze protein levels and more with an accurate, stable detection method. Learn how near-infrared fluorescence will dramatically improve the consistency and accuracy of your data.

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