Make More Discoveries with NIR Fluorescence Imaging
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Make More Discoveries

With Near-Infrared Fluorescence Imaging

Constantly Watching the Clock?

Direct fluorescence detection gives you high-quality images without the subjectivity or hassles of multiple film exposures.

Wish You Could Get Better Sensitivity?

Unmask subtle changes between samples with near-infrared detection. See the difference with the highest sensitivity.

Dealing with Quantitation Issues?

Confidently analyze publication-quality data with accurate, reproducible imaging.

Do More with Your Time

Get immediate results with stable signals

Image whenever you’re ready, with stable fluorescent signals. Unlike enhanced chemiluminescence (ECL) detection, timing and enzyme kinetics don’t affect your results. Forget taking multiple exposures loaded with variability and inconsistency. With fluorescence imaging, you can even re-image the same blot months later and see the same results.

Fluorescent signals are stable and unaffected by timing, so you can compare band intensities with confidence.

Does your Western blot detection method affect quantitative analysis?

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Detect multiple targets on the same blot

With multiplex fluorescence, you can detect two protein targets in each sample lane, with great sensitivity in both fluorescence channels. Consistency and reproducibility are improved because you can account for lane-to-lane variation in loading and transfer without stripping and re-probing.

700nm
800nm
700nm and 800nm Western Blot

Courtesy of Dr. Julien Courchet of the Polleux lab,
Columbia University Medical Center

See the Difference with the
Highest Sensitivity

View subtle changes with NIR imaging

In the visible spectrum, detection sensitivity is limited by high autofluorescence of blotting membranes, plastics, and biological materials. At near-infrared (NIR) wavelengths, however, low background fluorescence means high sensitivity for your blots. The high sensitivity of NIR helps you detect subtle changes between samples, giving you more confidence in your data.

Visible region (Vy3) High autofluorescence Neasr-infrared region (700nm) Low autofluorescence

Discover More with
Quantitative Westerns

Analyze the most accurate data

You ask big questions, and your detection method should be able to handle all the answers. In immunoblot imaging, your signals are directly proportional to the amount of protein over the linear dynamic range (LDR) of the detection system. Capture all your data accurately the first time, every time with the wide linear dynamic range of Odyssey® near-infrared fluorescence detection. See all your data accurately to answer your toughest questions.

“For studies reporting semi-quantitative analyses of immunoblots, authors should clearly explain how quantitative data were obtained…Note that some detection methods including detection of enhanced chemiluminescence using X-ray film have a very limited linear range.”

Instructions for authors. J. Biol. Chem. (2015).

Capture and analyze all of your data, without image saturation. Capture and analyze all of your data, without image saturation. Capture and analyze all of your data, without image saturation. Capture and analyze all of your data, without image saturation. Capture and analyze all of your data, without image saturation. Capture and analyze all of your data, without image saturation.

Odyssey imaging systems (blue) have the widest linear dynamic range (6 logs) for capturing the most quantitative data. Other detection systems, like film (orange) or typical CCD imagers (purple), have limited linear dynamic ranges of 1.5 logs and 4 logs, respectively.

Take Your Research Further

Take advantage of precise, reproducible imaging technology from the leaders in quantitative immunoblotting. Over 9,000 scientific publications reference Odyssey® imagers. Get the best, most reproducible Western blot data from stable NIR fluorescence detection.

"Verifiable and reproducible data is essential to support downstream discovery....When scientists cannot verify or reproduce basic and pre-clinical data, research dollars are squandered and discovery is delayed."

Bonnette, S. A Western blot and immunoprecipitation assay to verify antibody specificity.
BioTechniques. 59(3):168-169 (2015).


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