Get clearer answers with accurate, reproducible Western blots and more
Get consistent, reproducible digital images, without the hassles and unpredictability of film – so you can analyze your data right away and plan your next experiment.
See both strong and faint bands clearly in the same image, with great sensitivity and no image saturation. Multiplex with two fluorescent colors to see even more data.
Capture all the detail and complexity of your data. With 6 logs of linear dynamic range, your results are more reproducible. Near-infrared (NIR) fluorescence delivers consistent signals that aren’t affected by timing.
Skip the mess and inconsistency of film, substrates, and the darkroom. Direct near-infrared fluorescence gives you high-quality images without the subjectivity or hassles of multiple film exposures.
Film, chemiluminescent substrates, and darkroom expenses eat up resources that you could be using to move your research forward. Film processing chemicals and rinse waters are hazardous wastes that require special disposal. And a single X-ray film processor consumes an average of 788,000 gallons of water per year.1, 2
“The equipment is versatile and easy to use. It saved my group a lot of money as compared to traditional Western blotting.”
Clifford Nwaeburu, German Cancer Research Center, SelectScience® Review
Analyze your data right away – it’s already in digital format. One digital file contains all your image data. Record-keeping is easy and multiple exposures are a thing of the past.
To image and analyze many samples efficiently, use the Odyssey CLx to scan up to 9 mini-blots, 6 microplates, or 30 slides at the same time.
Cell-based assays, protein arrays, gel shift assays, tissue section imaging, and more are at your fingertips with near-infrared fluorescence.
“We are finding that we can use the Odyssey CLx for just about everything protein-related we do in the lab.”
Dr. Jeremy Chambers, Florida International University
In-Cell Western™ Assay
NIR Fluorescent Western Blot
EMSA / Gel Shift Assay
Image courtesy of C. Kearn, University of Washington
One digital image file contains all your data. See both strong and faint bands clearly in a single image, without image saturation, “blowout”, or sacrificing sensitivity.
Increase the consistency and reproducibility of your results by capturing a single image, rather than comparing multiple exposures captured under variable conditions.
“The superior dynamic range and sensitivity allows me to confidently report my data.”
Lars Engstrom, Mirati Therapeutics, SelectScience® Review
High signal-to-noise ratios give you more confidence in your data, and can help you reliably detect subtle changes between samples. Low background fluorescence at near-infrared (NIR) wavelengths means high sensitivity for your blots. In the visible spectrum, detection sensitivity is limited by high autofluorescence of blotting membranes, plastics, and biological materials.
Powerful, precise laser excitation and specialized optics are the keys to high signal-to-noise ratios and outstanding image quality. Other imagers use LEDs or diffuse white light sources that provide weak, non-specific excitation light and limit sensitivity.
Near-infrared fluorescent signals are stable and reproducible over time. Timing and enzyme kinetics don’t affect your results, so you can image your blots when it’s convenient for you. You can even re-image the same blot later and see the same results.
With multiplex fluorescence, you can detect two protein targets in each sample lane, with great sensitivity in both fluorescence channels. Consistency and reproducibility are improved because you can account for lane-to-lane variation in loading and transfer without stripping and re-probing.
“The infrared imaging system allows detection of multiple proteins on a single blot, without the need for stripping and re-probing.”
Bond et al (2008) Biol Proced Online. 10: 20–28.
Digital fluorescence shows your strong signals as they really are. Film and CCD imagers only show you part of the picture, because data are lost when images become saturated. To see the whole picture, you need enough capacity (dynamic range) to consistently document your strongest bands.
“When saturated, film exposures can also hide sample-to-sample variations in high-abundance proteins.”
Janes (2015) Science Signaling 8(371): rs2
You ask big questions, and your detection method should be able to handle all the answers. Now you can capture all of your data in a single image. Strong and faint bands are accurately documented in just one scan by the Odyssey CLx Imager.
With an unprecedented 6 logs of linear dynamic range, you’ll never lose data because of image saturation. Other detection methods can’t handle the challenge and let some of your data slip away.
“The ability to quantitate those bands is just amazing!”
Dr. Wendy L. Picking, Associate Professor, Oklahoma State University
Your Western blot data should reflect real changes in your samples, not the timing of your detection. Stable near-infrared fluorescent signals give you consistent, reproducible answers – no matter when you image.
But chemiluminescent detection and other enzyme/substrate methods are “moving targets”. Signals are dynamic and constantly changing, with inconsistent enzyme kinetics across the blot. Data output is heavily dependent on timing, so relative comparisons of band intensity may give very different results for different time points. The answer you get depends on when you ask.
With NIR fluorescence imaging, you can be more confident that the changes you see on your Western blots reflect actual changes in the biochemistry and composition of your samples.
“Just because we can put numbers on an image does not imply that we should.”
Janes (2015) Science Signaling 8(371): rs2
Do more with consistent, reproducible digital imaging and the flexibility to perform a variety of assays and experiments. See the benefits of stable NIR fluorescence, and detect multiple targets in the same lane. Get the most accurate results with over 6 logs of linear dynamic range, so you can make more discoveries.Request a Demo
“The brilliant signal-to-noise ratio in combination with the ability to truly quantify the data is really outstanding.”
Geir Bjørkøy, University College of Sør-Trøndelag