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Odyssey® CLx Imaging System

How It Works

 

Technology that helps you do more, see more, and discover more

Do More with Integrated Imaging and Analysis Software

With a few simple clicks, Image Studio™ software puts high-quality data at your fingertips. No more manual camera adjustments or multiple exposures. Image Studio saves your settings, so you only need to click once to capture an image next time. And it’s compatible with both Mac® and Windows® computers.

Ready to see what you discovered? Streamline your analysis with easy-to-use image view tools and convenient data analysis. Pre-set options to export images for publication or digital media let you present your findings faster.

image studio acquire

Do more with versatile image analysis tools

Image Studio’s acquisition, analysis, and formatting tools are presented in a simple ribbon interface. Use the Western Blot Analysis ribbon to quickly place lanes and band markers, quantify bands, and normalize your results. Additional ribbon tools streamline the analysis of microplate assays (including In-Cell Western™ cell-based assays), place grids for array analysis, and even analyze small-animal imaging data.

Introducing Image Studio

Free Image Studio Lite Download

Unsupported image editing and analysis software programs can compromise the integrity of your data, as they are not intended for Western blot image analysis. Image Studio™ Software never alters raw experimental data and is custom-built for Western blots. Always use Image Studio or Image Studio Lite software for image adjustments, quantitation, and annotation of data acquired on LI-COR imaging systems. See other best practices for image analysis.

Use AutoScan to see all of your data the first time, every time

Get a great image every time, without choosing or adjusting any imager settings. Image Studio’s AutoScan function provides the widest possible dynamic range in every image, while minimizing or eliminating image saturation. You see all of your data the first time you scan, without sacrificing sensitivity or detection of fainter bands.

Use AutoScan with your Odyssey CLx Imager to capture images with exactly the same settings every time, for the most consistent and reproducible results. Even new or occasional users can easily acquire high-quality images on the first try.

“The LI-COR protein detection system enabled determination of band intensity in Western blots over a greater dynamic range than that of conventional chemiluminescence.”

Wang et al (2007) Proc Natl Acad Sci 104(30):12365-12370.

Eliminate the guesswork and see all your signals the first time.

Eliminate the guesswork and see all your signals the first time.The AutoScan function captures all data points across the widest possible dynamic range. Saturation of strong signals is minimized, without loss of fainter signals.

Capture data across a very wide dynamic range.

Capture data across a very wide dynamic range. AutoScan ensures that you get the most sensitive, quantitative, complete dataset every time you capture an image. In this Western blot experiment, signals were linear across an 8000-fold range.

Discover more with a laser-based imager

The quality of excitation light also affects image quality and your ability to detect subtle changes. The Odyssey CLx Imager uses powerful, precise lasers for the most effective excitation of near-infrared (NIR) fluorescent dyes.

Other imagers use LEDs or diffuse white light sources that provide weak, non-specific excitation light and limit sensitivity. These inefficient light sources limit image quality and performance.

LI-COR laser-based imaging systems provide high performance at optimal NIR wavelengths. The Odyssey CLx Imager’s 700 nm and 800 nm fluorescence channels outperform other NIR fluorescence imagers, giving you the best sensitivity, image quality, and signal-to-noise ratios.

“[Quantitative fluorescent Western blotting] allows biologists to carry out comparative expression analysis with greater sensitivity and accuracy than ever before.”

Eaton et al (2014) J. Vis. Exp. (93): e52099, doi:10.3791/52099

Get the sensitivity and image quality you need to detect subtle changes.

Get the sensitivity and image quality you need to detect subtle changes. In typical imagers, excitation light comes from inefficient white light or LED sources. These sources can produce a broad range of wavelengths, and may generate only a small amount of the desired NIR excitation light. NIR lasers deliver powerful, precisely tuned excitation light to generate strong signals and provide superior image quality.

Who's Using It?

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  1. Anderson and Anderson (2002) Mol Cell Prot. 1:845–867.
  2. Bromage et al (2009) Mar Ecol Prog Ser. 376: 123–132.
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