- Anderson and Anderson (2002) Mol Cell Prot. 1:845–867.
- Bromage et al (2009) Mar Ecol Prog Ser. 376: 123–132.
Technology that helps you do more, see more, and discover more
With a few simple clicks, Image Studio™ software puts high-quality data at your fingertips. No more manual camera adjustments or multiple exposures. Image Studio saves your settings, so you only need to click once to capture an image next time. And it’s compatible with both Mac® and Windows® computers.
Ready to see what you discovered? Streamline your analysis with easy-to-use image view tools and convenient data analysis. Pre-set options to export images for publication or digital media let you present your findings faster.
Image Studio’s acquisition, analysis, and formatting tools are presented in a simple ribbon interface. Use the Western Blot Analysis ribbon to quickly place lanes and band markers, quantify bands, and normalize your results. Additional ribbon tools streamline the analysis of microplate assays (including In-Cell Western™ cell-based assays), place grids for array analysis, and even analyze small-animal imaging data.
Unsupported image editing and analysis software programs can compromise the integrity of your data, as they are not intended for Western blot image analysis. Image Studio™ Software never alters raw experimental data and is custom-built for Western blots. Always use Image Studio or Image Studio Lite software for image adjustments, quantitation, and annotation of data acquired on LI-COR imaging systems. See other best practices for image analysis.
Get a great image every time, without choosing or adjusting any imager settings. Image Studio’s AutoScan function provides the widest possible dynamic range in every image, while minimizing or eliminating image saturation. You see all of your data the first time you scan, without sacrificing sensitivity or detection of fainter bands.
Use AutoScan with your Odyssey CLx Imager to capture images with exactly the same settings every time, for the most consistent and reproducible results. Even new or occasional users can easily acquire high-quality images on the first try.
“The LI-COR protein detection system enabled determination of band intensity in Western blots over a greater dynamic range than that of conventional chemiluminescence.”
Wang et al (2007) Proc Natl Acad Sci 104(30):12365-12370.
The quality of excitation light also affects image quality and your ability to detect subtle changes. The Odyssey CLx Imager uses powerful, precise lasers for the most effective excitation of near-infrared (NIR) fluorescent dyes.
Other imagers use LEDs or diffuse white light sources that provide weak, non-specific excitation light and limit sensitivity. These inefficient light sources limit image quality and performance.
LI-COR laser-based imaging systems provide high performance at optimal NIR wavelengths. The Odyssey CLx Imager’s 700 nm and 800 nm fluorescence channels outperform other NIR fluorescence imagers, giving you the best sensitivity, image quality, and signal-to-noise ratios.
“[Quantitative fluorescent Western blotting] allows biologists to carry out comparative expression analysis with greater sensitivity and accuracy than ever before.”
Eaton et al (2014) J. Vis. Exp. (93): e52099, doi:10.3791/52099