Get clearer answers with accurate, reproducible Western blots and DNA gels.
Get consistent, reproducible digital images, without the hassles and unpredictability of film – so you can analyze your data right away and plan your next experiment.
Document DNA gels, image ECL blots, and detect multiple targets on the same blot with two channel near-infrared fluorescence. Capture clear, low-background images every time without adjusting camera settings.
Capture all the detail and complexity of your data. With 6 logs of linear dynamic range, your results are more reproducible. Near-infrared fluorescence delivers consistent signals that aren’t affected by timing.
Skip the mess and inconsistency of film, substrates, and the darkroom. Direct near-infrared fluorescence gives you high-quality images without the subjectivity or hassles of multiple film exposures.
Film, chemiluminescent substrates, and darkroom expenses eat up resources that you could be using to move your research forward. Film processing chemicals and rinse waters are hazardous wastes that require special disposal. And a single x-ray film processor consumes an average of 788,000 gallons of water per year.1, 2Get a Quote
Document your DNA gel and detect your chemiluminescent or NIR blots on the same imager, without juggling multiple instruments.
“Instead of getting two or three different instruments for imaging Western blots, agarose gels, SDS-PAGE gels, and other assays, we purchased a single instrument that can be used for all of these applications.”
Rick Page, Miami University
Quantitative NIR Western Blot
Chemiluminescent Western Blot
Nucleic Acid Gel Documentation
Coomassie Protein Gel Documentation
See both strong and faint bands clearly in a single image, without image saturation, “blowout”, or sacrificing sensitivity.
Increase the consistency and reproducibility of your results by capturing a single image, without comparing multiple exposures or changing camera settings. The patented FieldBrite™ XT2 optics that power the Odyssey Fc allow you to get the best image every time.
“…digitally acquired images provide the more accurate representation of band intensity and its relationship to sample abundance.”
Janes (2015) Science Signaling 8(371): rs2
NIR fluorescent signals are stable and reproducible over time. Timing and enzyme kinetics don’t affect your results, so you can image your blots when it’s convenient for you. You can even re-image the same blot later and see the same results.
With multiplex fluorescence, you can detect two protein targets in each sample lane, with great sensitivity in both fluorescence channels.
Consistency and reproducibility are improved because you can account for lane-to-lane variation in loading and transfer without stripping and re-probing.
“The infrared imaging system allows detection of multiple proteins on a single blot, without the need for stripping and re-probing.”
Bond et al (2008) Biol Proced Online. 10: 20–28.
Digital fluorescence shows your strong signals as they really are. Film and CCD imagers only show you part of the picture, because data is lost when images become saturated. To see the whole picture, you need enough capacity (dynamic range) to consistently document your strongest bands.
“When saturated, film exposures can also hide sample-to-sample variations in high-abundance proteins...”
Janes (2015) Science Signaling 8(371): rs2
You ask big questions, and your detection method should be able to handle all the answers. Now you can capture all of your data in a single image. Strong and faint bands are accurately documented in just one scan by the Odyssey Fc Imager.
With an unprecedented 6 logs of linear dynamic range, you’ll never lose data because of image saturation. Other detection methods can’t handle the challenge and let some of your data slip away.
“Odyssey was the only one that came close to accurately measuring 10-fold serial dilutions of a protein sample over 3 orders of magnitude.”
Robin Rylaarsdam, Benedictine University, via SelectScience®
In an ideal world, your CCD imager would deliver a single, accurate image without settings to optimize or post-capture image processing.
However, other CCD imagers require you to manually adjust exposures to optimize the signal-to-noise ratio (SNR), which can lead to variability. Some systems also manipulate image data to artificially create the ideal exposure, correct for non-uniform image illumination, or artificially improve SNR.
On the other hand, the Odyssey Fc captures uniform, low-background images that are not subjected to image correction. Because post-capture image manipulation is not required, the Odyssey Fc provides superior data reproducibility.
Do more with consistent, reproducible digital imaging. Capture your blots in clear, low-background images every time without adjusting camera settings. Get the most accurate results from 6 logs of linear dynamic range for both near-infrared fluorescence and chemiluminescence, so you can make more discoveries.Request a Demo
“The Odyssey Fc imager is great in terms of ease of use and quality of data, and also helps us conserve bench space.”
Dr. Rick Page, Miami University