Find Accurate Answers from Westerns and Gels with the Odyssey Fc

Odyssey® Fc
Imaging System

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Get clearer answers with accurate, reproducible Western blots and DNA gels.

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Do More by Going Digital

Get consistent, reproducible digital images, without the hassles and unpredictability of film – so you can analyze your data right away and plan your next experiment.

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See More with Additional Channels
and Clearer Images

Document DNA gels, image ECL blots, and detect multiple targets on the same blot with two channel near-infrared fluorescence. Capture clear, low-background images every time without adjusting camera settings.

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Discover More with Richer, More Complete Data

Capture all the detail and complexity of your data. With 6 logs of linear dynamic range, your results are more reproducible. Near-infrared fluorescence delivers consistent signals that aren’t affected by timing.

Do More by Going Digital

Do more with your time

Skip the mess and inconsistency of film, substrates, and the darkroom. Direct near-infrared fluorescence gives you high-quality images without the subjectivity or hassles of multiple film exposures.

 

Typical times shown.

Simplify your protocol. Save time and minimize the number of manual steps that can introduce variability.

Do more with your funding

Film, chemiluminescent substrates, and darkroom expenses eat up resources that you could be using to move your research forward. Film processing chemicals and rinse waters are hazardous wastes that require special disposal. And a single x-ray film processor consumes an average of 788,000 gallons of water per year.1, 2

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Save thousands in ongoing costs with Odyssey Fc.
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Save thousands in ongoing costs with Odyssey Fc.

Save thousands in ongoing costs with Odyssey Fc. Digital imaging eliminates typical darkroom expenses like processor chemicals, developer maintenance, and x-ray film.

See More with Additional
Channels and Clearer Images

See more with more channels

Document your DNA gel and detect your chemiluminescent or NIR blots on the same imager, without juggling multiple instruments.

“Instead of getting two or three different instruments for imaging Western blots, agarose gels, SDS-PAGE gels, and other assays, we purchased a single instrument that can be used for all of these applications.”

Rick Page, Miami University

Quantitative NIR Western Blot

Quantitative NIR Western Blot

Chemiluminescent Western Blot

Chemiluminescent Western Blot

Nucleic Acid Gel Documentation

Nucleic Acid Gel Documentation

Coomassie Protein Gel Documentation

Coomassie Protein Gel Documentation

See the full story in a single image

See both strong and faint bands clearly in a single image, without image saturation, “blowout”, or sacrificing sensitivity.

Increase the consistency and reproducibility of your results by capturing a single image, without comparing multiple exposures or changing camera settings. The patented FieldBrite™ XT2 optics that power the Odyssey Fc allow you to get the best image every time.

“…digitally acquired images provide the more accurate representation of band intensity and its relationship to sample abundance.”

Janes (2015) Science Signaling 8(371): rs2

Odyssey Fc Western Blot image sequence 1 Odyssey Fc Western Blot image sequence 2 Odyssey Fc Western Blot image sequence 3 Odyssey Fc Western Blot image sequence 4 Odyssey Fc Western Blot image sequence 5 Odyssey Fc Western Blot image sequence 6 Odyssey Fc Western Blot image sequence 7 Odyssey Fc Western Blot image sequence 8 Odyssey Fc Western Blot image sequence 9 Odyssey Fc Western Blot image sequence 10 Odyssey Fc Western Blot image sequence 11 Odyssey Fc Western Blot image sequence 12 Odyssey Fc Western Blot image sequence 13 Odyssey Fc Western Blot image sequence 14 Odyssey Fc Western Blot image sequence 15 Odyssey Fc Western Blot image sequence 16 Odyssey Fc Western Blot image sequence 17 Odyssey Fc Western Blot image sequence 18 Odyssey Fc Western Blot image sequence 19 Odyssey Fc Western Blot image sequence 20

Show different views by adjusting the image display

One image file contains the full range of your data. Choose the best image display for further analysis, based on your protein of interest. Changing the image display settings never alters your raw data or signal intensities.

See your bands reliably with stable fluorescent signals

NIR fluorescent signals are stable and reproducible over time. Timing and enzyme kinetics don’t affect your results, so you can image your blots when it’s convenient for you. You can even re-image the same blot later and see the same results.

Fluorescent signals are stable and unaffected by timing, so you can compare band intensities with confidence.
Fluorescent signals are stable and unaffected by timing, so you can compare band intensities with confidence. NIR fluorescent signals are stable for months, because no enzymes or substrates are used. Direct detection is performed using secondary antibodies labeled with IRDye® NIR fluorescent dyes, or other commercially-available dyes. You’ll typically lose signal in less than an hour with ECL substrates and indirect enzymatic detection.

See multiple targets on the same blot

With multiplex fluorescence, you can detect two protein targets in each sample lane, with great sensitivity in both fluorescence channels.

Consistency and reproducibility are improved because you can account for lane-to-lane variation in loading and transfer without stripping and re-probing.

“The infrared imaging system allows detection of multiple proteins on a single blot, without the need for stripping and re-probing.”

Bond et al (2008) Biol Proced Online. 10: 20–28.

700nm
800nm
700nm and 800nm Western Blot
Multiplex to detect two different protein targets in each sample lane. Use secondary antibodies labeled with spectrally-distinct NIR fluorescent dyes to get more data from your blot. View, adjust, and analyze your results as a merged image, or as separate 700 nm and 800 nm channel images in pseudo-color or grayscale.

See your strong bands accurately

Digital fluorescence shows your strong signals as they really are. Film and CCD imagers only show you part of the picture, because data is lost when images become saturated. To see the whole picture, you need enough capacity (dynamic range) to consistently document your strongest bands.

“When saturated, film exposures can also hide sample-to-sample variations in high-abundance proteins...”

Janes (2015) Science Signaling 8(371): rs2

 

Get deeper data capacity with digital fluorescence. The Odyssey Fc Imager gives you a stunningly wide linear dynamic range – in other words, a very deep data capacity. Film has a shallow data capacity and cannot accurately record strong signals. Once that capacity is reached, all additional signal (read: your data) is simply lost. Many CCD imagers are also limited by data capacity and image saturation.

Discover More with Richer,
More Complete Data

Detect and analyze all your bands, faint and strong

You ask big questions, and your detection method should be able to handle all the answers. Now you can capture all of your data in a single image. Strong and faint bands are accurately documented in just one scan by the Odyssey Fc Imager.

With an unprecedented 6 logs of linear dynamic range, you’ll never lose data because of image saturation. Other detection methods can’t handle the challenge and let some of your data slip away.

“Odyssey was the only one that came close to accurately measuring 10-fold serial dilutions of a protein sample over 3 orders of magnitude.”

Robin Rylaarsdam, Benedictine University, via SelectScience®

Capture and analyze all of your data, without image saturation. Capture and analyze all of your data, without image saturation. Capture and analyze all of your data, without image saturation. Capture and analyze all of your data, without image saturation. Capture and analyze all of your data, without image saturation. Capture and analyze all of your data, without image saturation.

Capture and analyze all of your data, without image saturation. When your detection method has the capacity to collect all of your data, you can detect the differences between strong bands. Digital near-infrared fluorescence reveals the complete picture. With film you can’t tell when saturation begins, and it’s different in every exposure. Strong bands are all similarly dark, and densitometry can’t show you the differences. When a CCD imager reaches saturation (blue pixels), the results are inaccurate and not suitable for analysis (dotted line).

Get the right image in one scan, every time

In an ideal world, your CCD imager would deliver a single, accurate image without settings to optimize or post-capture image processing.

However, other CCD imagers require you to manually adjust exposures to optimize the signal-to-noise ratio (SNR), which can lead to variability. Some systems also manipulate image data to artificially create the ideal exposure, correct for non-uniform image illumination, or artificially improve SNR.

On the other hand, the Odyssey Fc captures uniform, low-background images that are not subjected to image correction. Because post-capture image manipulation is not required, the Odyssey Fc provides superior data reproducibility.

LI-COR: Complete Data Capture Binning Data Loss

Other CCD imagers artificially improve signal-to-noise (SNR), but sacrifice image resolution. “Binning” is a software algorithm that combines groups of pixels to create an image with improved SNR, but reduced image resolution. Because the Odyssey Fc is designed to collect low-background images, binning is not necessary and faint bands can be detected at the CCD’s full resolution.

Odyssey Fc Imager

See the Odyssey Fc in action

Do more with consistent, reproducible digital imaging. Capture your blots in clear, low-background images every time without adjusting camera settings. Get the most accurate results from 6 logs of linear dynamic range for both near-infrared fluorescence and chemiluminescence, so you can make more discoveries.

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“The Odyssey Fc imager is great in terms of ease of use and quality of data, and also helps us conserve bench space.”

Dr. Rick Page, Miami University

How It Works

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  1. US Environmental Protection Agency. Laboratories for the 21st century: best practices. Office of Administration and Resources Management. DOE/GO-102005-2008 (2005).
  2. Cohen, R et al. Making every drop work: increasing water efficiency in California’s commercial, industrial, and institutional sector. Natural Resources Defense Council (NRDC) Issue Paper (2009).
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