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Wide Linear Dynamic Range

wide liner range

Figure 1. Strong and weak bands are accurately detected in a single image without signal saturation. Serial dilutions of purified transferrin (10 ng to 1.2 pg) were detected with rabbit anti-transferrin and IRDye® 800CW secondary antibody. Acquisition time with the Odyssey Fc Imager was 2 minutes.

The wide dynamic range of the Odyssey® Fc System allows both strong and weak chemiluminescent or near-infrared fluorescent bands to be clearly captured with only one exposure.

Unique to all Odyssey platforms is the use of infrared laser excitation that out-performs visible fluorescence systems. In the visible wavelength range used by most fluorescence imagers, membranes and plastics produce high background due to light scattering and autofluorescence. This limits the sensitivity of visible fluorescence systems and makes it difficult to detect low abundance proteins at endogenous levels without saturation of stronger bands.

wide liner range grap

Figure 2. Fluorescent signals on the triplicate blots were quantified with the Image Studio software. Errors bars are shown, but are very small. Results were linear across the entire range tested (4,000-fold: 3.6 orders of magnitude).

This is especially valuable if you wish to quantify the signal within its available linear range. LI‑COR® FieldBrite™ XT2 technology compensates for time and substrate signal variance issues common to chemiluminescence methods and offers one-button image acquisition for both detection methods.

The accuracy and linearity of detection on the Odyssey Fc system allow you to be confident about differences in protein levels.

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