Equip your in vivo imaging needs with a system that adapts to your workflow and gives you reproducible data. Explore how the FieldBrite™ Xi2 technology at the heart of the Pearl Trilogy Small Animal Imaging System makes it all possible.
When you are devising innovative approaches to monitor and treat disease, your groundbreaking research demands high-sensitivity imaging data.
In vivo optical imaging using visible light is not sufficient to meet your sensitivity needs. Components of animal tissue, such as hemoglobin, absorb and scatter incident excitation light of most visible wavelengths. This limits light penetration, resulting in even lower fluorescence emission signals that are difficult to detect consistently.
Additionally, autofluorescence from tissue components increases background noise, yielding low signal-to-noise ratios.
Get superior sensitivity using the near-infrared detection of Pearl Trilogy’s FieldBrite Xi2 optical system.
In the NIR spectrum (680 nm to 820 nm), light absorbance by, and autofluorescence from, tissue components is significantly lower. As a result, animal tissue is rendered virtually transparent to excitation light in these wavelengths, allowing better penetration to detect deeper targets.
Fluorescence emission signals are also higher, with low background interference from biochemical components, which greatly enhances detection sensitivity. This means you can detect smaller and deeper subcutaneous, orthotopic, and non-palpable tumors, earlier.
Higher signal-to-noise ratios help you detect, track, validate and quantitate in vivo biochemical activity with accuracy and confidence.
Tissue autofluorescence is much lower at NIR wavelengths. Untreated nude mouse was imaged with visible (532 nm) and NIR (700 nm or 800 nm) light. Autofluorescence at 532 nm was very high.
Tracking biological distribution and clearance of dye-conjugated probes and agents? For every periodic measurement within your longitudinal study, you want consistent data.
White light and LED illumination generate excitation light in a wide wavelength range. Even with appropriate excitation and emission filters, some leakage of excitation light occurs, causing high background on images. In addition, light from these sources diffuses, resulting in variable illumination intensities across the imaging area. Uneven illumination results in uneven detection signals, leading to spatial variation in data, especially farther away from the light source.
Pearl Trilogy is engineered differently, keeping your imaging needs in mind. The FieldBrite Xi2 system couples NIR lasers with filter technology that delivers sharp excitation light within narrow wavelengths. Light leakage is significantly reduced, yielding low background and improved sensitivity to detect deeper targets.
Additionally, laser-based illumination sources are stable, causing minimal diffusion across the imaging field (CVs <3%). So, you get coherent data for your time course studies, accurately recording biological changes in samples.
A mouse organ (brain) was imaged 8x in variable locations using FieldBrite Xi2 technology. Move the slider to see the organ in each location. Note the CV is <3% across the entire field of view. Data is for illustrative purposes only.
Investigating in vivo uptake and localization of dye-conjugated imaging agents? To accurately measure your strongest and weakest signals, you’ll need an imaging system with a wide dynamic range of detection.
Imagers with limited dynamic ranges are unable to simultaneously detect signals differing more than 3 or 4 logs (1000 to 10,000X). As a result, images are saturated in areas of high signal intensity, and data are no longer coherent or quantitative. Correction usually involves manual adjustment of camera exposure before every image capture.
Don’t be limited by your imager’s dynamic range. Get the advantage of FieldBrite Xi2 technology’s industry-leading 6 logs of dynamic range. The system enables you to simultaneously capture the full range of signals without saturation, across samples and time points.
With Pearl Trilogy’s camera optics you don’t ever need to adjust your camera settings, so you can have complete confidence in your in vivo data, scan after scan.