Figure 1. pAkt and ß-Tubulin antibody specificity depends on blocker selection. In 5% BSA, the pAkt antibody shows extra bands (green); in I-Block™ (Applied Biosystems), ß-Tubulin shows extra bands. In Odyssey Blocker (PBS), antibody specificity is increased.
LI-COR offers several blocking buffers for use with the Odyssey® and Aerius Systems. All are optimized for use with IRDye® products and other near-infrared fluorophores and provide excellent performance for quantitative Western blots and other immunoassays.
Blocker choice is important for immunoassay success. Blocking buffers enhance sensitivity by reducing background interference, increasing signal-to-noise ratio, and promoting specific binding of the primary antibody while minimizing non-specific interactions.