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Casein Blocking Buffer

  • Ready-to-use formulation in PBS
  • Milk-based blockers may contain endogenous:
    • Biotin, which could interfere with biotin-streptavidin detection
    • Phospho-proteins, which could interfere with phospho-specific antibodies
Odyssey Buffer

Figure 1. Detection of p53 in serial dilutions of A431 lysate (5 µg to 78 ng). Casein was used for blocking and antibody dilution. Primary Ab: mouse anti-p53 (Sigma P6874); secondary Ab: IRDye 800CW Goat-anti-mouse (LI-COR 926-32210).

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