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Odyssey® Blocking Buffer (PBS)

  • Contains no mammalian proteins, for the lowest cross-reactivity with mammalian antibodies
  • Ready-to-use formulation in PBS
  • Excellent choice for biotin-streptavidin detection
  • Suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays
  • Also available in kits with Immobilon®-FL PVDF membranes

For larger pack sizes, please contact your local sales office.

Detection of ERK in serial dilutions of NIH/3T3 lysate

Figure 1. Detection of ERK in serial dilutions of NIH/3T3 lysate. Serial dilutions of NIH/3T3 lysate (156 ng/well → 10 µg/well) on Immobilon®-FL PVDF membrane were probed with rabbit anti-Erk (Santa Cruz P/N sc-94), detected with IRDye® 800CW Goat anti-Mouse IgG (LI-COR P/N 926-32210), and then scanned on Odyssey® CLx (auto scan 700 & s800).



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