Get a free sample of 4X Protein Sample Loading Buffer when you purchase Odyssey Blocking Buffer.
Some restrictions may apply. Valid only in specific countries.
Product Ordering Information
£41.00pack insert | msds/sds +wishlist
£129.00pack insert | msds/sds +wishlist
£339.00pack insert | msds/sds +wishlist
£949.00pack insert | msds/sds +wishlist
£498.00| msds/sds +wishlist
£1,375.00| msds/sds +wishlist
For larger pack sizes, please contact your local sales office.
Figure 1. Example of Detection of pAkt and total Akt in Calyculin A-treated Jurkat lysate using Odyssey Blocking Buffer (TBS) as blocking agent. pAkt and total Akt were detected in calyculin A-stimulated (+) and non-stimulated (-) Jurkat lysate at 10 µp;g; 5 µp;g; and 2.5 µp;g/well. Blot was blocked with Odyssey Blocking Buffer (TBS) and probed pAkt Rabbit mAb (Santa Cruz P/N sc-13560) and Akt mAb (CST P/N 2967). Signal was detected with IRDye® 800CW Goat anti-Rabbit IgG (LI-COR P/N 926-32211) and IRDye 680RD Goat anti-Mouse IgG (LI-COR P/N 926-68070) and scanned on Odyssey® CLx Imager (auto scan 700 & 800).